Innovative Fe(IV)-Triggered Chemiluminescence Assay for Rapid and Selective Detection of Total Phenolic Content

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL Analytical Chemistry Pub Date : 2025-03-22 DOI:10.1021/acs.analchem.5c00552

Huixin Shao, Chengyu Gu, Huajie Li, Ling Chen, Xiaohong Guan

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Abstract

Total phenolic content reflecting the overall concentration of phenolics in water is a valuable indicator for evaluating water quality. However, current total phenolic content quantification technologies are unsatisfactory due to their complexity, time-consuming nature, limited reliability, and low selectivity. To overcome these problems, we utilized the high reactivity and selectivity of tetravalent iron (Fe(IV)) toward phenolics to develop a surrogate method for total phenolic content based on the quenching effect of phenolics on the chemiluminescence (CL) produced during the oxidation of naproxen (NAP) by Fe(IV) in the Fe(II)-activated periodate (Fe(II)/PI) process. Experimental results showed a strong linear relationship between the chemiluminescence quenching capacity (CLQC) values and total phenolic content in the Fe(II)/PI-NAP process. The high reactivity and superior selectivity of Fe(IV) toward phenolics enable rapid, highly sensitive, and robust anti-interference quantification of total phenolic content using the developed CL method. The limit of quantitation and limit of detection of the developed CL method for total phenolics determination were 1.34 and 0.40 μM, respectively, expressed as phenol equivalents. Finally, we validated the feasibility of using the CLQC value as a surrogate indicator for total phenolic content in various real water samples. This work introduces a novel method for quantifying total phenolic content by determining the CLQC value of water samples using the Fe(II)/PI-NAP process, offering a promising alternative for controlling the discharge of phenolics.

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用于快速、选择性检测总酚类物质含量的创新型铁（IV）触发化学发光测定法

总酚含量反映了水体中酚类物质的总体浓度，是评价水质的重要指标。然而，目前的总酚含量定量技术由于其复杂性、耗时性、有限的可靠性和低选择性而令人不满意。为了克服这些问题，我们利用四价铁（Fe(IV)）对酚类物质的高反应性和选择性，基于酚类物质在Fe（II）活化高碘酸盐（Fe(II)/PI）氧化萘普生（NAP）过程中对化学发光（CL）的猝灭作用，建立了总酚含量的替代方法。实验结果表明，Fe(II)/PI-NAP过程中化学发光猝灭能力（CLQC）值与总酚含量之间存在较强的线性关系。Fe（IV）对酚类物质的高反应性和优越的选择性使得使用所开发的CL方法可以快速，高灵敏度和强大的抗干扰定量总酚含量。以苯酚当量表示，本方法的定量限为1.34 μM，检出限为0.40 μM。最后，我们验证了CLQC值作为各种实际水样中总酚含量的替代指标的可行性。本文介绍了一种利用Fe(II)/PI-NAP工艺通过测定水样的CLQC值来定量总酚含量的新方法，为控制酚类物质的排放提供了一种有希望的替代方法。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.