A ratiometric fluorescence biosensor based on enzyme-cascade signal amplification technology for the detection of lincomycin

IF 6.3 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY Food Control Pub Date : 2025-09-01 Epub Date: 2025-04-07 DOI:10.1016/j.foodcont.2025.111344

Yiyao Liu, Menglong Liu, Jingyi Xiao, Yi Ren, Jinyang Li, Jiayan Gao, Litao Chen, Xue Gao

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Abstract

An enzyme-nanozyme cascade amplified ratio fluorescence (RF) biosensor based on prepared MnFe-layered double hydroxide nanosheets (Mn-Fe LDH) and alkaline phosphatase (ALP) has been developed for sensitive and accurate detection of lincomycin (LIN). In this strategy, Mn-Fe LDH can catalyze the o-phenylenediamine (OPD) oxidation to generate fluorescence 2, 3-diaminophenazine (DAP) with a fluorescence wavelength of 565 nm. This study utilized LIN aptamer-modified magnetic beads and alkaline phosphatase-labeled complementary strand hybridization to prepare the MBs-Apt@cDNA-ALP. The MBs-Apt@cDNA-ALP could recognize LIN and release cDNA-ALP from the surface of the magnetic beads. After magnetic separation, cDNA-ALP catalyzed the 2-phosphate ascorbic acid (AAP) conversion to ascorbic acid (AA). The redox reaction between AA and Mn-Fe LDH led to a decrease in DAP production and fluorescence intensity. At the same time, AA converted into dehydroascorbic acid (DHAA), which reacted with OPD to form a quinoline derivative (DFQ) that emitted fluorescence at 435 nm. The constructed LIN biosensor exhibited a detection range of 1.125–250 nM with a limit of detection (LOD) of 0.0492 nM. Moreover, the RF aptasensor can eliminate background interference, deliver remarkable signal variations, and effectively analyze the LIN concentration in grass carp and shrimp samples.

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基于酶级联信号放大技术的比例荧光生物传感器检测林可霉素

以制备的mnfe层状双氢氧化物纳米片（Mn-Fe LDH）和碱性磷酸酶（ALP）为材料，建立了一种酶-纳米酶级联放大比例荧光（RF）生物传感器，用于灵敏、准确地检测林可霉素（LIN）。在该策略中，Mn-Fe LDH可以催化邻苯二胺（OPD）氧化生成荧光2,3 -二氨基吩嗪（DAP），荧光波长为565 nm。本研究利用LIN适配体修饰磁珠和碱性磷酸酶标记互补链杂交制备MBs-Apt@cDNA-ALP。MBs-Apt@cDNA-ALP可以识别LIN并从磁珠表面释放dna - alp。经磁分离后，dna - alp催化2-磷酸抗坏血酸（AAP）转化为抗坏血酸（AA）。AA与Mn-Fe LDH之间的氧化还原反应导致DAP的生成和荧光强度下降。同时，AA转化为脱氢抗坏血酸（DHAA），与OPD反应生成喹啉衍生物（DFQ），在435 nm处发出荧光。所构建的LIN生物传感器检测范围为1.125 ~ 250 nM，检出限为0.0492 nM。此外，射频感应传感器可以消除背景干扰，提供显著的信号变化，有效地分析草鱼和虾样品中的LIN浓度。

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来源期刊

Food Control 工程技术-食品科技

CiteScore

12.20

自引率

6.70%

发文量

758

审稿时长

33 days

期刊介绍： Food Control is an international journal that provides essential information for those involved in food safety and process control. Food Control covers the below areas that relate to food process control or to food safety of human foods: • Microbial food safety and antimicrobial systems • Mycotoxins • Hazard analysis, HACCP and food safety objectives • Risk assessment, including microbial and chemical hazards • Quality assurance • Good manufacturing practices • Food process systems design and control • Food Packaging technology and materials in contact with foods • Rapid methods of analysis and detection, including sensor technology • Codes of practice, legislation and international harmonization • Consumer issues • Education, training and research needs. The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.