[An improved lactose-peptone medium for the cultivation of chlorine-damaged E. coli bacteria].

R Schubert, F Helm
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Abstract

A description of a casein-soya-lactose broth (CSLB) for the cultivation of chlorine-impaired E. coli bacteria is given. In this liquid medium the recovery rates of chlorine impaired E. coli are superior or at least equal to recovery rates observed when casein-soya-broth (CSB) is used. Differences are regularly seen when lactose-pepton-broth (LPB) according the German Standards (DEV.K6) is used between direct inoculation into a liquid enrichment medium and inoculation into the same medium following membrane filtration are no longer found when CSSL-broth is used instead of LPB.

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一种改良的乳糖-蛋白胨培养基,用于培养被氯破坏的大肠杆菌。
介绍了一种培养氯损伤大肠杆菌的酪蛋白-大豆-乳糖肉汤(CSLB)。在这种液体培养基中,氯损伤大肠杆菌的回收率优于或至少等于使用酪蛋白-大豆肉汤(CSB)时所观察到的回收率。当根据德国标准(DEV.K6)使用乳糖-蛋白胨肉汤(LPB)直接接种到液体富集培养基中与在膜过滤后接种到同一培养基中时,通常可以看到差异,而当使用cssl肉汤代替LPB时,则不再发现差异。
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