Chengyuan Wu , Xinjie Wang , Ling Guo , Xingxu Huang , Lina Wu , He Huang
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引用次数: 8
Abstract
Selective and sensitive determination of ochratoxin A (OTA) is critical due to its high toxicity and wide distribution. Here, a neoteric electrochemical biosensor based on an exonuclease III (Exo III)-assisted recycling reaction triggering the trans-cleavage ability of CRISPR-Cas12a was developed, allowing the specific and ultrasensitive detection of OTA. In this strategy, the Exo III-assisted recycling reaction was used to convert and magnify the target recognition affair into the trigger DNA chain to the trans-cleavage activity of Cas12a for detecting electrochemical signal changes, thus enhancing detection sensitivity. When the Fc-ssDNA was fixed onto a glassy carbon electrode and used as a reporter probe under optimized conditions, the method showed good selectivity and sensitivity for OTA determination, and a detection limit of 0.74 fg/mL. Furthermore, the established method could sensitively identify OTA in spiked rice and peanut samples, demonstrating its application potential for practical detection.
期刊介绍:
Food Control is an international journal that provides essential information for those involved in food safety and process control.
Food Control covers the below areas that relate to food process control or to food safety of human foods:
• Microbial food safety and antimicrobial systems
• Mycotoxins
• Hazard analysis, HACCP and food safety objectives
• Risk assessment, including microbial and chemical hazards
• Quality assurance
• Good manufacturing practices
• Food process systems design and control
• Food Packaging technology and materials in contact with foods
• Rapid methods of analysis and detection, including sensor technology
• Codes of practice, legislation and international harmonization
• Consumer issues
• Education, training and research needs.
The scope of Food Control is comprehensive and includes original research papers, authoritative reviews, short communications, comment articles that report on new developments in food control, and position papers.