{"title":"[Histochemistry of mucosubstances. Technical considerations].","authors":"C A Pagnini, L Bozzola","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>A report concerning the histochemical methods used to differentiate mucosubstances and certain technical modifications made with the aim of obtaining better results : 1) rapid passage in a solution at pH1 after alcian blue reaction in order to ensure staining of sulfate mucosubstances only ; 20 plunging of sections first in FeCl for 10 minutes by the H.I.D. method ; 3) in the methylation reaction the optimum time is 12 hours.</p>","PeriodicalId":75498,"journal":{"name":"Annales d'anatomie pathologique","volume":"25 3","pages":"261-5"},"PeriodicalIF":0.0000,"publicationDate":"1980-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Annales d'anatomie pathologique","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
A report concerning the histochemical methods used to differentiate mucosubstances and certain technical modifications made with the aim of obtaining better results : 1) rapid passage in a solution at pH1 after alcian blue reaction in order to ensure staining of sulfate mucosubstances only ; 20 plunging of sections first in FeCl for 10 minutes by the H.I.D. method ; 3) in the methylation reaction the optimum time is 12 hours.
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