Annales d'anatomie pathologique最新文献

Oestradiol receptors were demonstrated using the technique of Sin Hang Lee. Receptors are seen after incubation of a section of breast tissue frozen immediately before, with oestradiol bound to a fluorescent molecule (17 beta-oestradiol-6-carboxymethyloxime-bovine serum albumin-fluorescein isothiocyanate). By the same technique, it is possible to visualise progesterone receptors (11 alpha-hydroxy-progesterone hemisuccinate-bovine serum albuminum-tetramethyl rhodamine isothiocyanate). This technique was applied routinely to all breast specimens sent to the laboratory. There were approximately 30 carcinomas, 10 fibroadenomas and 10 dysplasic lesions. Infiltrating carcinomas contained oestrogenic receptors, their distribution being heterogeneous. They appeared to consist of a mixture in varying proportions of positive and negative cells. Intracanalicular carcinomas contained little or no receptors. Receptors were present in Paget cells whilst surrounding keratinocytes contained none. Young fibroadenomas showed the presence of receptors within their epithelial and fibroblastic elements. By contrast, fibroadenomas in a state of involution were negative. Dysplasias may contain receptors, the quantity and distribution showing no typical pattern. In particular, intracanalicular epithelial proliferations may contain receptors. The results of this technique have not yet been correlated with those of "exchanges in vitro".

The authors report of a case of composite lymphoma, defined by the coexistence of two clearly different types of malignant lymphoma in a single lymph node or splenic site. A review of the literature led to the discovery of 20 cases of this type of lymphoma. The macroscopic and histological lesions are analysed.

A comparative light microscopy, histoenzymological and ultrastructural study enabled the authors, in a case of invasive lobular carcinoma of the breast, to emphasise certain special morphological traits of this tumour type and to make a contribution to the understanding of its histogenesis. By light microscopy, the only special features of the case were the abundance of mucus secreting tumour cells in the perilobular infiltrating zones and in the stromal texture with marked perigalactophoric hyalinosis and active elastic neogenesis. From an ultrastructural standpoint, intralobular malignant zones contained epithelial type cells, rich in microfilaments and with numerous desmosomal junctions. Two other cell types were identified at the periphery of the lobules. On consisted of round cells with intracytoplasmic cavities filled with mucus (signet ring cells). This group, in common with the intralobular cells, contained abundant amounts of alkaline phosphatase. The other consisted of elongated "pseudo-fibroblastic" cells, rich in microfibrils and in granular ergastoplasm with secretion at points of contact of collagen and elastin. These cells showed marked ATPase activity. They no doubt represented "hybrid" cells, intermediate in terms of their morphology and enzyme activity between myoepithelial and epithelial cells. These findings would thus appear to offer arguments in favour of the double cell origin -- duct and myoepithelial -- of lobular carcinoma.

In multinuclear cells of giant cell tumours of long bones studied in biopsies and histiotypic cultures the centrioles were found to be grouped at a distance from the nuclei, forming a giant centrosphere made of diplosomes in equal number to that of the nuclei. This arrangement renders the possibility of new mitotic activity highly unlikely. By contrast, close contacts between multinuclear cells and stromal cells together with the collapse of membranes between cells in contact and the centriolar arrangement represent arguments in favour of genesis by fusion from mononuclear cells of stromal type, in common with many polykaryons. The multinuclear cells of these tumours would appear to represent only the final stage in the evolution of certain mononuclear cells.

The authors by report a case of Brenner's tumour diagnosed during a hysterectomy carried out for a cervical carcinoma in situ in a menopausal patient with neoplastic adenopathy of unknown cause.

Comparative ultrastructural and histoenzymological study of 10 osteogenic (4 osteosarcomas), osteolytic (3 benign giant cell tumours, 2 malignant osteoclastomas) and fibrosarcomatous osseous tumours of the jaw emphasised the analogies between osteoblasts and fibroblastic stromal cells of giant cell tumours. These two cell types, with their abundant granular ergastoplasm, secrete a collagen interstitial fibrillary substance which is capable of secondary calcification. Their enzyme activities, with regard to oxidative mechanisms and protein synthesis are identical. Osteogenic activity as assessed by ATPase and alkaline phosphatase, more marked than in osteosarcomas, is also found in the stroma of benign giant cell tumours. With regard to the plasmodes characteristic of giant cell tumours, they are certainly original from both a morphological and enzymological standpoint : richness in mitochondria and lysosomes, intensity of oxidative reactions (B.O.H.B.D. +++). Nevertheless, the morphological appearances seen would be more in favour of their stromal origin than in their arising from monocytes-macrophages coming from the blood.

The participation of the sinusoidal elements in experimental iron-overloading has been studied. Ultrastructurally and microanalytically iron particles have been identified in Kupffer and Ito cells. The presence of iron particles in the Ito cells demonstrates its microphagocytic capacity.

During surgical cervical exploration of 30 patients with hyperparathyroidism (primary or secondary) 67 specimens have been collected: 22 adenomas, 2 glands of primary hyperplasia and 28 glands of secondary hyperplasia (in 7 patients). Fat content of parenchymal cells has been studied using Oil Red O stain on cryostat cut sections: this content is very low in hyperactive lands (adenomas, primary or secondary hyperplasias) and high in normal or suppressed glands, even enlarged. There is no correlation between parenchymal fat and stromal fat. Practical and theoretical implications of these results are discussed.