Paloma López , M. Tekesa Pérez Ureña , Ernesto García , M. Teresa Alda , Manolo Espinosa
{"title":"Detection of different types of receptors for deoxyribonucleic acid in competent Bacillus subtilis","authors":"Paloma López , M. Tekesa Pérez Ureña , Ernesto García , M. Teresa Alda , Manolo Espinosa","doi":"10.1016/S0323-6056(81)80100-0","DOIUrl":null,"url":null,"abstract":"<div><p>Competent cells of <em>Bacillus subtilis</em> are able to bind but not to take up DNA in magnesium-free medium. Wall-membrane complexes, but not cell walls, bind homologous DNA. Binding of DNA to membrane vesicles suggests that DNA-receptor sites are located at the membrane level. On the basis of competition experiments with homologous and heterologous DNAs, in cells and in membrane vesicles, different types of DNA receptors are detected.</p></div><div><p>Kompetente <em>Bacillus subtilis</em>-ZeMen sind in Mg-freiem Medium in der Lage, DNS zu binden, jedoch nicht aufzunehmen. Die Bindung an Membran-Vesikel weist darauf hin, daß DNS-Rezeptoren in der Membran lokalisiert sind. Bei Verwendung homologer und heterologer DNS konnten in Konkurrenz-Experimenten in Zellen und in Membran-Vesikeln unterschiedliche DNS-Rezeptoren festgestellt werden.</p></div>","PeriodicalId":76869,"journal":{"name":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abteilung: Mikrobiologie der Landwirtschaft der Technologie und des Umweltschutzes","volume":"136 6","pages":"Pages 524-532"},"PeriodicalIF":0.0000,"publicationDate":"1981-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0323-6056(81)80100-0","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Zentralblatt fur Bakteriologie, Parasitenkunde, Infektionskrankheiten und Hygiene. Zweite naturwissenschaftliche Abteilung: Mikrobiologie der Landwirtschaft der Technologie und des Umweltschutzes","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0323605681801000","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
Competent cells of Bacillus subtilis are able to bind but not to take up DNA in magnesium-free medium. Wall-membrane complexes, but not cell walls, bind homologous DNA. Binding of DNA to membrane vesicles suggests that DNA-receptor sites are located at the membrane level. On the basis of competition experiments with homologous and heterologous DNAs, in cells and in membrane vesicles, different types of DNA receptors are detected.
Kompetente Bacillus subtilis-ZeMen sind in Mg-freiem Medium in der Lage, DNS zu binden, jedoch nicht aufzunehmen. Die Bindung an Membran-Vesikel weist darauf hin, daß DNS-Rezeptoren in der Membran lokalisiert sind. Bei Verwendung homologer und heterologer DNS konnten in Konkurrenz-Experimenten in Zellen und in Membran-Vesikeln unterschiedliche DNS-Rezeptoren festgestellt werden.