Detection of different types of receptors for deoxyribonucleic acid in competent Bacillus subtilis

Paloma López , M. Tekesa Pérez Ureña , Ernesto García , M. Teresa Alda , Manolo Espinosa
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引用次数: 1

Abstract

Competent cells of Bacillus subtilis are able to bind but not to take up DNA in magnesium-free medium. Wall-membrane complexes, but not cell walls, bind homologous DNA. Binding of DNA to membrane vesicles suggests that DNA-receptor sites are located at the membrane level. On the basis of competition experiments with homologous and heterologous DNAs, in cells and in membrane vesicles, different types of DNA receptors are detected.

Kompetente Bacillus subtilis-ZeMen sind in Mg-freiem Medium in der Lage, DNS zu binden, jedoch nicht aufzunehmen. Die Bindung an Membran-Vesikel weist darauf hin, daß DNS-Rezeptoren in der Membran lokalisiert sind. Bei Verwendung homologer und heterologer DNS konnten in Konkurrenz-Experimenten in Zellen und in Membran-Vesikeln unterschiedliche DNS-Rezeptoren festgestellt werden.

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枯草芽孢杆菌不同类型脱氧核糖核酸受体的检测
我可以随意使用抗生素去除细菌细菌的所有污秽但却无法在麻碱中提取DNA并不是墙那边的以DNA捕获者的位置在薄膜层在接触homolous和异性恋DNA、囊囊和胚胎的技术实验基础上发现不同的DNA标本有能力的巴力毒肝细胞(zecilus)能够在控制核磁共振的反应中吸收dna,但无法融入网络。请留意,脱氧核糖核酸的受体位置,并不是完全准确的。使用荷马和直质脱氧核糖核酸(一种dna)的竞争实验,在细胞和薄膜内的细胞和细胞膜上,发现了不同的脱氧核糖核酸。
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