[Distribution of 1-[14C] GABA and its conjugates with nicotinate, pyridoxal phosphate and biotin in tissues of guinea pigs in closed systems].

Abstract

Distribution of the total radioactive label in the blood, different parts of the brain (the whole cortex and its lobes, bulbi olfactorii, hippocamp, striopallidum, hypothalamus, thalamus, peduculi cerebri, cerebellum, pons, medulla oblongata), eye (retina, hyaloid and vascular membrane), hypophysis and liver of guinea pig males has been studied after the hypodermic injection of [14C] GABA and products of its conjugation with pyridoxal phosphate, nicotinic acid and biotin in the equimolar dose (50 nmol/g) under normal conditions and in case of action of the closed volume factors. Accumulation of the total label in the hypophysis after injection of [14C] GABA, PLP-[14C] GABA and nicotinoyl-[14C] GABA was 3.6, 3.1 and 2.7 times (respectively) as much as that in the blood. The content of the labelled compounds in the cortical structures of the telencephalon and in the diencephalon was the greatest in comparison with other parts of the brain. The permeability through a hemato-ophthalmic barrier was 3-5 times as much as that through a hemato-encephalic barrier. The closed volume factors lead to higher accumulation of the label as compared with the control after injection of [14C] GABA, PLP-[14C] GABA and biotinyl-[14C] GABA.