5-Lipoxygenase activity in the human pancreas.

Journal of lipid mediators Pub Date : 1993-11-01
J A Mancini, C Li, P J Vickers
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Abstract

Low levels of 5-lipoxygenase (5-LO), the first committed enzyme in the synthesis of leukotrienes (LTs), have been reported in the porcine pancreas. We have quantitated 5-LO activity in subcellular fractions of pancreas samples from three human donors. 5-LO activity was detectable in all samples, although enzyme activity was lower than in human leukocytes. 5-LO in human pancreas samples displayed highest specific activity in membrane fractions, and did not require arachidonic acid (AA) addition for activity. These unusual characteristics of pancreatic 5-LO appear to be due, at least in part, to the presence of unesterified AA in the pancreas samples. Western blot analysis demonstrated that the human pancreas contains low levels of 5-lipoxygenase-activating protein (FLAP) in addition to 5-LO.

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5-脂氧合酶在人体胰腺中的活性。
低水平的5-脂氧合酶(5-LO),在合成白三烯(lt)的第一个承诺的酶,已报道在猪胰腺。我们已经定量测定了3个人类供体胰腺样本亚细胞部分的5-LO活性。虽然酶活性低于人类白细胞,但在所有样品中均可检测到5-LO活性。人体胰腺样品中的5-LO在膜组分中显示出最高的比活性,并且不需要添加花生四烯酸(AA)即可产生活性。胰腺5-LO的这些不寻常的特征似乎至少部分是由于胰腺样本中未酯化的AA的存在。Western blot分析表明,人胰腺除含有5-LO外,还含有低水平的5-脂氧合酶激活蛋白(FLAP)。
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