Y Okamoto, H Yoshida, M Ino, S Nakamura, G Okamoto, K Mizoguchi, Y Suzuki, J Sugatani, M Miwa
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引用次数: 0
Abstract
A factor responsible for releasing PAF produced in stimulated human polymorphonuclear leukocytes, which had been previously reported in human serum (Miwa et al. (1992) J. Immunol. 148, 872-880), was also confirmed to be present in inflammatory exudate. PAF-releasing factor, partially purified from human serum, was shown to possess higher affinity for PAF than for triacylglycerol, cholesterol ester, fatty acid or phosphatidylcholine. PAF bound to this factor aggregated washed rabbit platelets to the same extent as that bound to BSA, but was difficult to be hydrolyzed using PAF acetylhydrolase. These observations strongly suggest that PAF-releasing factor functions as a PAF carrier in blood and in the inflammatory response.
一种负责释放受刺激的人多形核白细胞产生的PAF的因子，以前曾在人血清中报道过(Miwa et al. (1992) J. Immunol. 148, 872-880)，也被证实存在于炎症渗出液中。部分从人血清中纯化的PAF释放因子对PAF的亲和力高于对甘油三酯、胆固醇酯、脂肪酸或磷脂酰胆碱的亲和力。与该因子结合的PAF与与BSA结合的PAF聚集洗涤兔血小板的程度相同，但难以用PAF乙酰水解酶水解。这些观察结果强烈提示PAF释放因子在血液和炎症反应中作为PAF载体起作用。
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