[Cryopreservation of embryos in the in vitro fertilization/embryo transfer program].

Ceskoslovenska gynekologie Pub Date : 1993-09-01
M Miková, L Zetová, T Mardesic, P Müller, A Stroufová
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引用次数: 0

Abstract

An ultra-rapid method for cryopreservation of human embryos, using 1,2-propane-diol, was developed. 280 spare embryos obtained in the IVF program were frozen by this method. 91 embryos were thawed and 68 of them (74.7%) survived the procedure with more than half the blastomeres intact. 65 embryos were transferred in 28 cycles. Two pregnancies developed (7.1%), one of which ended by the birth of a healthy girl (the first birth after transfer of a frozen/thawed embryo in the Czech Republic). The other pregnancy is ongoing.

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体外受精/胚胎移植项目中胚胎的低温保存。
建立了一种利用1,2-丙二醇超高速冷冻保存人类胚胎的方法。在体外受精项目中获得的280个备用胚胎用这种方法冷冻。91个胚胎被解冻,其中68个(74.7%)存活下来,一半以上的卵裂球完好无损。在28个周期内移植65个胚胎。发生了两次怀孕(7.1%),其中一次以健康女孩的出生结束(在捷克共和国移植冷冻/解冻胚胎后的第一次分娩)。另一个正在怀孕。
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