Transforming growth factor alpha (TGF-alpha) expression correlates with DNA replication in regenerating rat liver whatever the hepatectomy extent.

M Scotté, A Laquerrière, S Masson, M Hiron, P Ténière, J Hémet, J P Lebreton, M Daveau
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引用次数: 1

Abstract

Liver regeneration is a physiological mechanism that leads to restoration of the hepatic parenchyma following hepatectomy or toxic injury. As a two-third hepatectomy is the usual model to analyse this phenomenon, few studies have compared liver regeneration after minor vs major hepatectomy. We have used a quantitative RT-PCR technique to study the hepatic transcription of the TGF-alpha gene in rats submitted to 30% or 80% hepatectomy and we have correlated this transcription with the regenerative response assessed by flow cytometry and Ki-67 expression. The level of TGF-alpha expression and the regenerative response were different, according to the volume of liver removed, and were statistically correlated (r = 0.679, p = 0.002). TGF-alpha expression and phase S peaked at day 2 vs 6 in the 80% vs 30% hepatectomized rat groups, respectively. Ki-67 expression occurred at 2 h post-hepatectomy in the two groups of rats and was observed until day 14 mainly in the 80% hepatectomy group. Our results indicate that TGF-alpha expression in regenerating liver is strongly correlated with hepatocyte mitosis, that a delayed regenerative response occurs following 30% hepatectomy and that the course of regeneration differs between minor vs major hepatectomy.

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无论肝切除程度如何,转化生长因子α (tgf - α)表达与再生大鼠肝脏DNA复制相关。
肝再生是肝切除术或中毒性损伤后肝实质恢复的一种生理机制。由于三分之二肝切除术是分析这一现象的常用模型,很少有研究比较小肝切除术和大肝切除术后的肝脏再生。我们使用定量RT-PCR技术研究了接受30%或80%肝切除术的大鼠的tgf - α基因的肝脏转录,并将这种转录与流式细胞术和Ki-67表达评估的再生反应相关联。tgf - α表达水平与再生反应随肝脏切除量的不同而不同,且具有统计学相关性(r = 0.679, p = 0.002)。在80%和30%肝切除大鼠组中,tgf - α表达和S期分别在第2天和第6天达到峰值。Ki-67在两组大鼠肝切除术后2 h表达,主要在80%肝切除术组观察到14天。我们的研究结果表明,再生肝脏中的tgf - α表达与肝细胞有丝分裂密切相关,30%肝切除术后会出现延迟的再生反应,并且小肝切除术与大肝切除术的再生过程不同。
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Hepatitis C virus genotypes: epidemiological and clinical associations. Benelux Study Group on Treatment of Chronic Hepatitis C. Deleted form of hepatocyte growth factor (dHGF) increases the number of platelets in rats with liver cirrhosis. In vivo and in vitro hepatic 31P magnetic resonance spectroscopy and electron microscopy of the cirrhotic liver. Unusual spontaneous hepatic vein to paraumbilical vein shunt in a patient with Budd-Chiari syndrome and cirrhosis: a case report. Transforming growth factor alpha (TGF-alpha) expression correlates with DNA replication in regenerating rat liver whatever the hepatectomy extent.
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