{"title":"Location of regulatory gene in penicillin G acylase operon (pacR) of E. coli D816","authors":"Lan Jiang, Yucheng Wang, Shengli Yang","doi":"10.1016/S1050-3862(97)00011-9","DOIUrl":null,"url":null,"abstract":"<div><p>Regulatory gene in Penicillin G Acylase operon (pacR) of <em>E. coli</em> D816 was located in a <em>Taq</em>1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 2","pages":"Pages 51-54"},"PeriodicalIF":0.0000,"publicationDate":"1997-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(97)00011-9","citationCount":"9","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386297000119","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 9
Abstract
Regulatory gene in Penicillin G Acylase operon (pacR) of E. coli D816 was located in a Taq1-Dra1 fragment within the pac structure gene. Two ORFs were found in this fragment and their transcriptional orientations were opposite with pac. ORF2 was determined as pacR by point mutation.
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