Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA

Genetic analysis, techniques and applications Pub Date : 1999-12-15 DOI:10.1016/S1050-3862(99)00008-X

Jae-Sam Hwang , Jin-Sung Lee , Tae-Won Goo , Eun Young Yun , Hae-Ryong Sohn , Ho Rak Kim , O-Yu Kwon

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引用次数: 21

Abstract

The phylogenetic relationships between Bombycidae (Bombyx mori and Bombyx mandarina) and Saturniidae (Antheraea yamamai and Antheraea pernyi) were investigated based on large and small mitochondiral rRNA genes. About 430 bp of four kinds of PCR-amplified fragments were sequenced and aligned. For the 16S rRNA gene, B. mori shared a 98, 87 and 86% sequence homology with B. mandarina, A. yamamai and A. pernyi, and for the 12S rRNA gene, B. mori shared a 99, 89 and 88% sequence homology with B. mandarina, A. yamamai and A. pernyi, respectively. DNA sequence data were also used for a phylogenetic analysis. All of the trees showed monophyly for both Bombycidae and Saturniidae. The monophyly confidence limits of these trees were estimated using bootstrapping tests and measured more than 99% for all trees for both Bombycidae and Saturniidae.

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基于线粒体DNA编码大rna和小rna的家蝇科和家蝇科分子遗传关系

基于线粒体rRNA的大小基因，研究了家蚕科(家蚕和中国家蚕)和家蚕科(山蚕和柞蚕)的系统发育关系。对4种pcr扩增片段约430 bp进行测序和比对。家蚕16S rRNA基因同源性分别为98、87和86%，家蚕12S rRNA基因同源性分别为99、89和88%。家蚕16S rRNA基因同源性分别为98、87和86%。DNA序列数据也用于系统发育分析。所有的树都表现出家蚕科和家蚕科的单系性。利用bootstrapping试验估计了这些树种的单系置信限，结果表明，家蚕科和土蜂科所有树种的单系置信限均大于99%。

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Genetic analysis, techniques and applications

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