Expression of glycoprotein gp43 in stage-specific forms and during dimorphic differentiation of Paracoccidioides brasiliensis.

R Mattar-Filho, M O Azevedo, M Pereira, R S Jesuino, S M Salem-Izacc, W A Brito, J L Gesztesi, R B Soares, M S Felipe, C M Soares
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引用次数: 7

Abstract

Expression of the 43 kDa glycoprotein (gp43) was analysed in several Paracoccidioides brasiliensis isolates. Using one- and two-dimensional analysis of crude cellular extracts, it was shown that protein expression in yeast and mycelium was dependent on the isolate analysed. In two strains, in both yeast and mycelium cells. gp43 was present, whereas expression was restricted to the yeast phase of two other strains. The clinical implications of this phase-specific gp43 expression are uncertain.

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糖蛋白gp43在巴西副球虫阶段特异性形式和二形分化中的表达。
分析了43 kDa糖蛋白(gp43)在巴西副球虫分离株中的表达。利用粗细胞提取物的一维和二维分析,表明酵母和菌丝体中的蛋白质表达依赖于所分析的分离物。在两种菌株中,酵母和菌丝细胞中。Gp43存在,而其他两个菌株的表达仅限于酵母期。这种阶段特异性gp43表达的临床意义尚不确定。
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