A new technique of screening gene mutation—fluorescent fingerprinting

Abstract

A new technique called fluorescent fingerprinting (FF) was set up and used to screen and detect gene mutation from a large number of samples by using fluorescent labelled ddNTP and ABI-373A sequencer. By comparing the colour ladders of several lanes from the same sequencing region of different samples, any band with a different colour from its neighbours or a pattern displacement could be easily found out in the Macintosh screen which indicates a gene mutation. Then, the sequencing peak-chromatograms were made out only for the selected samples.