Construction and sequencing of new derivatives of the pIN-III-ompA secretion vector

Alan Fauconnier, Ary Van Elsen, Alex Bollen
{"title":"Construction and sequencing of new derivatives of the pIN-III-ompA secretion vector","authors":"Alan Fauconnier,&nbsp;Ary Van Elsen,&nbsp;Alex Bollen","doi":"10.1016/S1050-3862(98)00005-9","DOIUrl":null,"url":null,"abstract":"<div><p>The growing interest in proteins exported by bacteria led to the development of cloning vectors designed for targeting recombinant proteins to extracytoplasmic compartments. The pIN-III-<em>ompA</em> vector family fulfils such a function. Here we report the construction and sequencing of new pIN-III-<em>ompA</em> derivatives with alternate polylinker sites, increasing the cloning flexibility of these vectors.</p></div>","PeriodicalId":77142,"journal":{"name":"Genetic analysis, techniques and applications","volume":"14 4","pages":"Pages 129-131"},"PeriodicalIF":0.0000,"publicationDate":"1998-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S1050-3862(98)00005-9","citationCount":"5","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Genetic analysis, techniques and applications","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S1050386298000059","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 5

Abstract

The growing interest in proteins exported by bacteria led to the development of cloning vectors designed for targeting recombinant proteins to extracytoplasmic compartments. The pIN-III-ompA vector family fulfils such a function. Here we report the construction and sequencing of new pIN-III-ompA derivatives with alternate polylinker sites, increasing the cloning flexibility of these vectors.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
pIN-III-ompA分泌载体新衍生物的构建与测序
对细菌输出蛋白质的兴趣日益增长,导致了克隆载体的发展,旨在将重组蛋白靶向胞浆外区室。pIN-III-ompA矢量族实现了这样的功能。在这里,我们报道了新的pIN-III-ompA衍生物的构建和测序,这些衍生物具有交替的多联位点,增加了这些载体的克隆灵活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Molecular genetic relationships between Bombycidae and Saturniidae based on the mitochondria DNA encoding of large and small rRNA A reliable PCR amplification method for microdissected tumor cells obtained from paraffin-embedded tissue DNA region responsible for transcriptional regulation of the Escherichia coli penicillin amidase (pac) gene by CRP and PAA Site-directed insertion and insertion–deletion mutations in the Escherichia coli chromosome simplified Characterization of genetic defects of hemophilia A in mainland China
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1