The effect of taxifolin on experimental testicular ischaemia reperfusion injury in rats. A biochemical and histopathological analysis

IF 0.8 4区医学 Q4 ANDROLOGY Revista Internacional De Andrologia Pub Date : 2022-01-01 DOI:10.1016/j.androl.2020.05.008

Fevzi Bedir , Huseyin Kocaturk , Fatma Betul Ozgeris , Gulce Naz Yazici , Zeynep Suleyman , Halis Suleyman

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引用次数: 3

Abstract

Objectives

The aim of the study is to investigate the protective effect of taxifolin (3,5,7,3,4-pentahydroxy flavanone), a strong antioxidant, against testicular I/R injury in rats biochemically and histopathologically.

Materials and methods

50 mg/kg taxifolin was administered to taxifolin + testicular torsion–detorsion (TTTD, n-10) group of Albino Wistar male rats by oral gavage. Distilled water .5 ml as a solvent was administered to testicular torsion–detorsion (TTD, n-10) and Healthy Control (SG, n-10) groups using the same method. An hour after the administration of taxifolin and distilled water, anaesthesia (ketamine 60 mg/kg) was administered to all animal groups. TTD and TTTD group animals were subjected to testicular torsion at 720 degrees for four hours during anaesthesia. At the end of this period, testicular detorsion was applied and perfusion was allowed for four hours. Sham operation was applied to SG group.

Results

Our biochemical experiment results showed that the amount of malondialdehyde (MDA) in testicular tissue of TTD group presented a significant increase compared to SG and TTTD groups whereas total glutathione (tGSH) and superoxide dismutase (SOD) levels decreased. In addition, while TTD group presented severe histopathological damage in germinal epithelium cell and seminiferous tubule, mild damage was observed in TTTD group.

Conclusions

The results of our experiment indicate that taxifolin could be useful in the treatment of testicular I/R damage.

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杉木素对大鼠实验性睾丸缺血再灌注损伤的影响。生化和组织病理学分析

目的探讨强抗氧化剂紫杉醇(3,5,7,3,4-五羟基黄酮)对大鼠睾丸I/R损伤的生物化学和组织病理学保护作用。材料与方法将50 mg/kg的紫杉醇脂灌胃给紫杉醇脂+睾丸扭转-扭转(TTTD, n-10)组雄性白化Wistar大鼠。睾丸扭转-扭转组(TTD, n-10)和健康对照组(SG, n-10)用同样的方法给予蒸馏水0.5 ml作为溶剂。给药1 h后，各动物组均予氯胺酮60 mg/kg麻醉。TTD组和TTTD组在麻醉状态下进行睾丸720度扭转4小时。在这段时间结束时，施加睾丸扭曲并允许灌注4小时。SG组采用假手术治疗。结果与SG和TTD组相比，TTD组大鼠睾丸组织中丙二醛(MDA)含量显著升高，总谷胱甘肽(tGSH)和超氧化物歧化酶(SOD)水平降低。此外，TTD组在生发上皮细胞和精小管中出现了严重的组织病理学损伤，而TTD组则出现了轻度损伤。结论紫杉醇素对睾丸I/R损伤有一定的治疗作用。

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来源期刊

Revista Internacional De Andrologia ANDROLOGY-

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Revista Internacional de Andrología es la revista oficial de la Asociación Española de Andrología, Medicina Sexual y Reproductiva (ASESA), la Sociedade Portuguesa de Ardrologia, la Sociedad Argentina de Andrología (SAA), la Asociación Iberoamericana de Sociedades de Andrología (ANDRO), y la Federación Española de Sociedades de Sexología. La revista publicada trimestralmente es revisada por pares y es líder en el la especialidad y en español y portugués. Recientemente también publica artículos en inglés. El objetivo de la revista es principalmente la promoción del conocimiento y la educación médica continua, con un enfoque especial en el público español y latinoamericano, a través de la publicación de las contribuciones importantes de la investigación en el campo. Todos los miembros de las sociedades antes mencionadas reciben la revista y otros suscriptores individuales e institucionales de España, Portugal y América Latina.