{"title":"[Gene identification of mumps wild virus isolated firstly in Jilin province].","authors":"Shuang Wang, Xin Chang, Jian-Hui Zhou","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.</p><p><strong>Methods: </strong>Throat swabs were collected and to isolated mump virus with Vero/slam cells.Cytopathic effect was observed and determined mump virus type with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).</p><p><strong>Results: </strong>Of the total 15 throat swabs, different CPE were observed in 3 throat swabs but genotypes are same, they are F type, 3 throat swabs were not observed cytopathic effect but genotypes were F type.</p><p><strong>Conclusion: </strong>There were different kinds of cytopathic effect in one mump outbreak although genotypes were same.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2009-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To observe Cytopathic Effect of wild-type mump viruses in Vero/SLAM cell and identify genotype in a mump outbreak in 2008.
Methods: Throat swabs were collected and to isolated mump virus with Vero/slam cells.Cytopathic effect was observed and determined mump virus type with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Results: Of the total 15 throat swabs, different CPE were observed in 3 throat swabs but genotypes are same, they are F type, 3 throat swabs were not observed cytopathic effect but genotypes were F type.
Conclusion: There were different kinds of cytopathic effect in one mump outbreak although genotypes were same.
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