{"title":"[Optimization of method of titration rabies virus with fluorescence focus units assay].","authors":"Miao Chai, Ge-lin Xu, Wen Sun","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.</p><p><strong>Methods: </strong>The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.</p><p><strong>Results: </strong>Data generated indicateed that a significant correlation between the two methods (r=99).</p><p><strong>Conclusion: </strong>This method is simple and rapid. It will be considered as a useful alternative method to the LD50.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: A new Fluorescence Focus Units (FFU) developed for Rabies Virus titration replace current reference method (LD50) so as to reduce the cost.
Methods: The BSR cell was infected by triple serial dilution of the Rabies Virus CTN strain, then detect the fluorescent focus by FITC labeling anti-Rabies Virus monoclonal antibodies. All the samples were assayed in both FFU and LD50.
Results: Data generated indicateed that a significant correlation between the two methods (r=99).
Conclusion: This method is simple and rapid. It will be considered as a useful alternative method to the LD50.
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