{"title":"[Establishment of one-step reverse transcription-polymerase chain reaction method for mumps virus hydrophobin gene detection].","authors":"Xin Chang, Jian-Hui Zhou, Shuang Wang","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).</p><p><strong>Methods: </strong>To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.</p><p><strong>Results: </strong>At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.</p><p><strong>Conclusions: </strong>The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To establish a kind of simple method for identifying mumps virus gene: one-step reverse transcription-polymerase chain reaction, RT-PCR).
Methods: To design primer in both sides of SH gene, and then to do the sensitivity and specificity test for the RT-PCR method.
Results: At least 10(0.7) TCID50 mumps virus can be detected by this method. 5 strains of mumps virus can be detected and 4 strains of non-mumps virus including measles and rubella virus can't be detected by this method.
Conclusions: The one-step RT-PCR method is a rapid and simple method for identifying mumps virus gene.
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