{"title":"[Gene characteristic of VP1 region of poliovirus isolates from acute flaccid paralysis in Hebei Province].","authors":"Mei Chen, Dong-Mei Yan, Hui Zhu","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>In order to provide the evidence for polio free status, the VP1 gene characteristic analysis of poliovirus isolated from acute flaccid paralysis (AFP) cases and close contacts were conducted in Hebei Province during 2007-2008.</p><p><strong>Methods: </strong>Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.</p><p><strong>Result: </strong>The total number of poliovirus isolates were 42. Most isolates were type II and III. No wild poliovirus and vaccine derived poliovirus (VDPVs) was detected.</p><p><strong>Conclusion: </strong>Gene characteristic of VP1 region of poliovirus can be used to identify the polio virus transmission and guide the work of immunization program.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2010-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: In order to provide the evidence for polio free status, the VP1 gene characteristic analysis of poliovirus isolated from acute flaccid paralysis (AFP) cases and close contacts were conducted in Hebei Province during 2007-2008.
Methods: Virus isolation and identification were conducted according to the 4th edition of WHO polio laboratory manual. The sequence of VP1 region were amplified and sequenced. The phylogenetic trees based on VP1 region were constructed.
Result: The total number of poliovirus isolates were 42. Most isolates were type II and III. No wild poliovirus and vaccine derived poliovirus (VDPVs) was detected.
Conclusion: Gene characteristic of VP1 region of poliovirus can be used to identify the polio virus transmission and guide the work of immunization program.
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