{"title":"[Application of a simple method for detection of rubella virus genome by loop-mediated isothermal amplification (LAMP)].","authors":"Shuang Wang, Jian-hui Zhou, Xiang Hou","doi":"","DOIUrl":null,"url":null,"abstract":"<p><strong>Objective: </strong>To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).</p><p><strong>Method: </strong>Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.</p><p><strong>Results: </strong>The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.</p><p><strong>Conclusion: </strong>RT-LAMP is more simple and convenient than RT-PCR.</p>","PeriodicalId":56402,"journal":{"name":"中国疫苗和免疫","volume":"15 6","pages":"518-20"},"PeriodicalIF":0.0000,"publicationDate":"2009-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"中国疫苗和免疫","FirstCategoryId":"1085","ListUrlMain":"","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To use a new simple Reverse Transcription Loop-mediated isothermal amplification (RT-LAMP) method was applied to detect rubella virus nucleic acid and compared with Reverse Transcription-Polymerase Chain Reaction (RT-PCR).
Method: Comparing the detection rate of the RT-LAMP method with that of RT-PCR for detecting rubella virus nucleic acid from rubella virus.
Results: The nucleic acid positive rates of all 11 strains of rubella virus were 100% by the two methods, the positive rate was 55%.
Conclusion: RT-LAMP is more simple and convenient than RT-PCR.
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