[Norovrius genotype I.8 being detected from sporadic cases of acute gastroenteritis in Nanning municipal].

Abstract

Objective: To study the genic characteristic of Norovrius genotype I.8 from sporadic gastroenteritis of a hospital in Nanning Municipal.

Methods: Specimens of diarrhea patients from a hospital in Nanning Municipal between January 2007 and December 2008 were collected and detected for Noroviral genogroup Iribonucleic acid (RNA) by real-time reverse transcription-polymerase chain reaction(real-time RT-PCR). All positive specimens were subjected for sequencing analysis and constructed phylogenetic tree.

Results: Norovirus genogroup I RNA were detected in 3 of 696 stool specimens. The homology between one of the four strains (NN07230) and reference strain of G I.8 (WUG I/00/JP) was 94.5%; and the homology between the NN07230 strain and strains circulating during 2006-2007 in Japan and Korea ranged from 96.7% to 98.5%. And they formed one cluster in the phylogenetic tree.

Conclusion: The research showed that genotype I.8 infection exists in sporadic gastroenteritis in Nanning City.