[Application of nonaqueous capillary electrophoresis for chiral pharmaceutical and biomedical analysis ].

Abstract

The potentialities of nonaqueous capillary electrophoresis for chiral analysis were demonstrated through many pharmaceutical and biomedical applications, such as the stereoselective assay of acidic and basic drugs in plasma and urine as well as in vitro metabolism studies. A fundamental aspect of the quality control of chiral drugs in single-isomer forms, i.e. the enantiomeric purity determination, was also investigated. Moreover, the mechanisms of intermolecular interactions involved in the chiral separations observed in nonaqueous systems were elucidated using nuclear magnetic resonance.