Effects of different extracts of Eugenia caryophyllata on pentylenetetrazole-induced seizures in mice.

Abstract

Objective: To investigate the possible anticonvulsant effect of different extracts of Eugenia caryophyllata (clove) on pentylenetetrazole (PTZ)-induced seizures in mice.

Methods: The animals were divided into saline, 50, 100, 250 and 500 mg/kg of aqueous extract, 50, 100, 250 and 500 mg/kg of ethanolic extract, and 50, 100, 250 and 500 mg/kg of chloroformic extract of clove groups. The extracts or saline were injected 60 min before each PTZ injection. Latency to the first minimal clonic seizure (MCS) and generalized tonic-clonic seizure (GTCS) and the percent of mortality were recorded.

Results: Aqueous extract of clove at doses of 50, 100, 250 and 500 mg/kg significantly extended the MCS and GTCS latency (P<0.05). The MCS latency in mice treated with 50, 100 and 250 mg/kg of the ethanolic extract was significantly increased (P<0.05). The GTCS latency in mice treated with 50, 100, 250 and 500 mg/kg of ethanolic extract was significantly higher than that of the saline-treated group (P<0.05). There were no significant differences in MCS and GTCS latency between mice treated with different chloroformic extract of clove or saline.

Conclusion: The aqueous and ethanolic extracts of clove could inhibit the PTZ-induced convulsion, and this plant has the potential to be used as a new therapeutic agent for control of seizures. The exact mechanisms and the active compounds that are responsible for the anticonvulsive effect need to be clarified in future studies.