{"title":"Biological responses of xenobiotic metabolizing enzymes to lead exposure in cultured H4IIE rat cells.","authors":"Wageh Sobhy Darwish, Yoshinori Ikenaka, Mayumi Ishizuka","doi":"","DOIUrl":null,"url":null,"abstract":"<p><p>This study was undertaken to investigate the constitutive response of xenobiotic metabolizing enzymes (XMEs) to lead (Pb2+) exposure in cultured rat liver (H4IIE) cell lines. Phase I enzymes such as CYP1A1 and CYP1A2 had mRNA expressions that were slightly induced after exposure to low concentrations of Pb2+; however, under higher concentrations of Pb2+, the mRNA expressions of CYP1A1 and CYP1A2 were significantly down-regulated. These effects were in correspondence with AhR mRNA expression. Phase II enzymes had mRNA expressions that were reduced upon exposure to Pb2+. Metallothionein mRNA expression was induced after treatment with Pb2+ in a dose-dependent trend. In conclusion, Phase I and II enzymes were significantly modulated upon lead exposure indicating some toxicological implications for lead exposure in cultured H4IIE cells.</p>","PeriodicalId":56285,"journal":{"name":"Japanese Journal of Veterinary Research","volume":null,"pages":null},"PeriodicalIF":0.4000,"publicationDate":"2013-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Japanese Journal of Veterinary Research","FirstCategoryId":"97","ListUrlMain":"","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"VETERINARY SCIENCES","Score":null,"Total":0}
引用次数: 0
Abstract
This study was undertaken to investigate the constitutive response of xenobiotic metabolizing enzymes (XMEs) to lead (Pb2+) exposure in cultured rat liver (H4IIE) cell lines. Phase I enzymes such as CYP1A1 and CYP1A2 had mRNA expressions that were slightly induced after exposure to low concentrations of Pb2+; however, under higher concentrations of Pb2+, the mRNA expressions of CYP1A1 and CYP1A2 were significantly down-regulated. These effects were in correspondence with AhR mRNA expression. Phase II enzymes had mRNA expressions that were reduced upon exposure to Pb2+. Metallothionein mRNA expression was induced after treatment with Pb2+ in a dose-dependent trend. In conclusion, Phase I and II enzymes were significantly modulated upon lead exposure indicating some toxicological implications for lead exposure in cultured H4IIE cells.
期刊介绍：
The Japanese Journal of Veterinary Research (JJVR) quarterly publishes peer-reviewed articles on all aspects of veterinary science. JJVR was originally published as a “University Journal” of veterinary science at Hokkaido University from more than 60 years ago. Currently, JJVR, is Japan’s leading scientific veterinary journal, and provides valuable information for the development of veterinary science by welcoming contributions from researchers worldwide.
JJVR offers online submission for Regular Papers, Short Communications, and Review Articles that are unpublished and not being considered for publication elsewhere. Research areas include:
Anatomy, Physiology, Biochemistry, Pharmacology, Microbiology, Infectious diseases, Parasitology, Laboratory Animal Science and Medicine, Internal Medicine, Surgery, Pathology, Theriogenology, Molecular Medicine, Public Health, Radiation Biology, Toxicology, Wildlife Biology and Medicine, Veterinary Hygiene, The other fields related to veterinary science.
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