Japanese Journal of Veterinary Research最新文献

英文中文

Inactivation of sars-cov-2 by povidone-iodine products: Implications for effective mouth rinsing and gargling 聚维酮碘产品灭活sars-cov-2:对有效漱口和漱口的影响

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2021-08-01 DOI: 10.14943/jjvr.69.3.183

H. Kariwa, H. Sawa, Shintaro Kobayashi

Coronavirus disease 2019 (COVID-19) is caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). SARS-CoV-2 is transmitted mainly via droplets and contact. The implementation of infection control measures is important to reduce the number of COVID-19 cases. Thus, the ability of several povidone-iodine (PVP-I) products to inactivate SARS-CoV-2 was evaluated based on their in vitro inactivation efficacy. PVP-I solutions such as Isodine Gargle® (ethical and consumer products), Isodine Gargle C®, and Isodine Nodo Fresh® for 30 or 60 s decreased the viral infectivity level from 2-4 × 106 TCID50/ml to below the detectable level (> 99.9% reduction). Our results indicate that the use of Isodine® mouthwash and gargle products is an effective infection control measure against SARS-CoV-2.

2019冠状病毒病（新冠肺炎）是由严重急性呼吸综合征冠状病毒2（SARS-CoV-2）引起的。严重急性呼吸系统综合征冠状病毒2型主要通过飞沫和接触传播。实施感染控制措施对于减少新冠肺炎病例数量至关重要。因此，基于几种聚维酮碘（PVP-I）产品的体外灭活效力，评估了其灭活严重急性呼吸系统综合征冠状病毒2型的能力。PVP-I溶液，如Isodine Gargle®（伦理和消费品）、Isodine Gargle C®和Isodine Nodo Fresh®，持续30或60秒，将病毒感染水平从2-4×106 TCID50/ml降低到可检测水平以下（降低>99.9%）。我们的研究结果表明，使用Isodine®漱口水和漱口产品是对抗严重急性呼吸系统综合征冠状病毒2型的有效感染控制措施。

引用次数: 4

Can procalcitonin be used as a clinical biomarker during bacterial, viral and parasitic infections in dogs? 降钙素原能否作为犬类细菌、病毒和寄生虫感染的临床生物标志物?

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2021-02-01 DOI: 10.14943/JJVR.69.1.5

E. Matur, B. Dokuzeylül, Mukaddes Özcan, H. Çetinkaya, Murat Arslan, E. Or, Songül Erhan, Ü. Çötelioğlu

The aim of this study is to investigate the usability of serum procalcitonin level in dogs as a clinical biomarker for the distinction between bacterial, viral, and parasitic diseases. A total of 160 dogs were used. The animals were evaluated in four groups as control and those with bacterial, viral, and parasitic infections. Serum procalcitonin, tumor necrosis factor-alpha (TNF- α ), interleukin-1 beta (IL-1 β ), interleukin-6 (IL-6), interferon-gamma (IFN- γ ) level, and total white blood cell (WBC), and differential leukocyte count were determined. Serum procalcitonin level was found to be higher in dogs with bacterial infection compared to the control group and dogs with viral disease ( P = 0.019). It was observed that serum procalcitonin level in dogs with bacterial infection varies related to the disease agent and it was found higher in those with pyometra ( P = 0.009). Serum procalcitonin level was higher in dogs which had parasitic infections but, the difference is not significant. IL-6 level was found higher in dogs with viral and parasitic diseases than those in the control ( P = 0.006). A negative correlation was determined between procalcitonin and IFN- γ ( P = 0.001). While a positive correlation was detected between procalcitonin and WBC count, a negative correlation was determined between procalcitonin and monocyte percentage. In conclusion, serum procalcitonin level can be used as a clinical biomarker in bacterial diseases and, perhaps, in some parasitic diseases in dogs. However, further studies should be conducted to determine threshold values that take the severity of infection, its prevalence, and clinical course into account.

本研究的目的是研究犬血清降钙素原水平作为区分细菌性、病毒性和寄生性疾病的临床生物标志物的可用性。总共使用了160只狗。这些动物被分为四组进行评估，分别是对照组和细菌、病毒和寄生虫感染组。测定血清降钙素原、肿瘤坏死因子- α (TNF- α)、白细胞介素-1 β (IL-1 β)、白细胞介素-6 (IL-6)、干扰素- γ (IFN- γ)水平，以及白细胞总数(WBC)和差异白细胞计数。细菌感染犬的血清降钙素原水平高于对照组和病毒感染犬(P = 0.019)。细菌感染犬血清降钙素原水平与疾病病原相关，脓脓病犬血清降钙素原水平较高(P = 0.009)。寄生虫感染犬血清降钙素原水平较高，但差异不显著。病毒和寄生虫病犬IL-6水平高于对照组(P = 0.006)。降钙素原与IFN- γ呈负相关(P = 0.001)。降钙素原与白细胞计数呈正相关，降钙素原与单核细胞百分比呈负相关。综上所述，血清降钙素原水平可作为细菌性疾病和某些寄生虫病的临床生物标志物。然而，应该进行进一步的研究，以确定考虑感染严重程度、患病率和临床病程的阈值。

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引用次数: 3

Development of a highly sensitive method for the detection of Cryptosporidium parvum virus type 1 (CSpV1) 小隐孢子虫1型病毒高灵敏检测方法的建立

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2020-08-01 DOI: 10.14943/JJVR.68.3.159

A. Shehata, H. Bando, Y. Fukuda, Mohammad Hazzaz Bin Kabir, F. Murakoshi, M. Itoh, A. Fujikura, Hiroaki Okawa, Takuto Endo, A. Goto, Masayuki Kachi, Toshie Nakayama, Yuto Kano, Shoko Oishi, K. Otomaru, Kei Kazama, M. Essa, Kentaro Kato

Cryptosporidium is an apicomplexan zoonotic parasite that infects most mammals, including humans. Cryptosporidium parvum virus type 1 (CSpV1) is the first member within the Partitiviridae family recognized to infect protozoan hosts. Cryptosporidium tracking based on CSpV1 detection has been attempted; however, each study used different conditions for the PCR protocol, primers, and target viral sequences. Accordingly, the sensitivity of PCR-based CSpV1 detection remains unclear. In addition, oocyst purification from clinical samples can be problematic due to small number of oocysts, sample degradation and low yield efficiency of currently used purification methods. Here we show that the second half of the coding region of dsRNA2 can be detected from various types of clinical samples, without the need for oocyst purification, by using a semi-nested-PCR technique. Furthermore, we show that the short sequence targeted in this study has higher diversity than the Cryptosporidium GP60 gene. Taken together, our findings suggest that this method could be used as an important tracking marker for Cryptosporidium species.

隐孢子虫是一种复杂的人畜共患寄生虫，感染大多数哺乳动物，包括人类。细小隐孢子虫病毒1型（CSpV1）是Partitiviridae家族中第一个被识别感染原生动物宿主的成员。已经尝试了基于CSpV1检测的隐孢子虫追踪；然而，每项研究都对PCR方案、引物和靶病毒序列使用了不同的条件。因此，基于PCR的CSpV1检测的灵敏度仍不清楚。此外，由于目前使用的纯化方法卵囊数量少、样品降解和产率低，从临床样品中纯化卵囊可能存在问题。在这里，我们表明，通过使用半嵌套PCR技术，可以从各种类型的临床样本中检测dsRNA2编码区的后半部分，而不需要纯化卵囊。此外，我们发现本研究中靶向的短序列比隐孢子虫GP60基因具有更高的多样性。总之，我们的发现表明，这种方法可以作为隐孢子虫物种的重要跟踪标记。

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引用次数: 1

Clinical efficacy of the combined treatment of anti-PD-L1 rat-bovine chimeric antibody with a COX-2 inhibitor in calves infected with Mycoplasma bovis 抗pd - l1大鼠-牛嵌合抗体联合COX-2抑制剂治疗牛支原体感染犊牛的临床疗效

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2020-05-01 DOI: 10.14943/JJVR.68.2.77

S. Goto, S. Konnai, Yuki Hirano, J. Kohara, T. Okagawa, N. Maekawa, Y. Sajiki, Kei Watari, Erina Minato, Atsuhi Kobayashi, S. Gondaira, H. Higuchi, M. Koiwa, M. Tajima, E. Taguchi, M. Ishida, R. Uemura, S. Yamada, M. Kaneko, Y. Kato, Keiichi Yamamoto, Mikihiro Toda, Yasuhiko Suzuki, S. Murata, K. Ohashi

引用次数: 8

NMR based serum metabolomics for monitoring newborn preterm calves'health 基于核磁共振的血清代谢组学监测新生儿早产儿的健康状况

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2020-05-01 DOI: 10.14943/JJVR.68.2.105

A. Basoglu, N. Başpınar, Cristina Licari, L. Tenori, A. Naseri

It was aimed to detect the novel future biomarkers using a metabolomics approach in premature calves. Calves born previous to 270 days' pregnancy are at risk, and the earlier the calving is, the higher the risk. More trials are needed in neonatology field as it little known almost the generally metabolic status of preterm neonates. To date, this is the first NMR (nuclear magnetic resonance) based study on serum metabolomics at set intervals in premature calves. Biochemical health profiles and NMR based metabolomic analysis were performed in twenty-five premature dairy calves. The whole animals partly recovered following 72h. Clinical data were compatible with those of premature animals. Increased levels of AST and CPK may be attributed to subclinic trauma at birth. Alterations in metabolites, increases in 3-hydroxybutyrate, citrate, leucine and isoleucine at 48th and 72h; choline, formate, fatty acids and polyunsaturated fatty acids at 72h, and valine at 48h; and decreases in myo-inositol at 48h and 72h were meaningful for monitoring the recovery at a molecular level in premature calves. Metabolomics became an important tool for identification of premature calves’ clinical pathology and monitoring therapeutic picture.

其目的是利用代谢组学方法在早产小牛中检测新的未来生物标志物。怀孕270天前出生的小牛有风险，而且产犊越早，风险越高。由于对早产儿的总体代谢状况知之甚少，需要在新生儿学领域进行更多的试验。迄今为止，这是第一个基于NMR(核磁共振)的早产儿血清代谢组学研究。对25头早产儿进行了生化健康概况和基于核磁共振的代谢组学分析。72h后全鼠部分恢复。临床数据与早产动物一致。AST和CPK水平升高可能归因于出生时的亚临床创伤。代谢物的变化，3-羟基丁酸盐、柠檬酸盐、亮氨酸和异亮氨酸在第48和72小时增加;72h胆碱、甲酸酯、脂肪酸和多不饱和脂肪酸，48h缬氨酸;而肌醇在48和72小时的下降，在分子水平上对监测早产犊牛的恢复有意义。代谢组学已成为鉴定早产犊牛临床病理和监测治疗情况的重要工具。

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引用次数: 0

Review on counter measures to coronavirus disease 2019 (COVID-19) pandemic, May 2020 2019冠状病毒病(COVID-19)大流行应对措施综述，2020年5月

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2020-01-01 DOI: 10.14943/JJVR.68.3.133

Taishi Kidaka, S. Lokupathirage, B. D. S. Muthusinghe, B. Lombe Pongombo, Christida E Wastika, Zhouxing Wei, Shizuka Yoshioka, M. Ishizuka, Y. Sakoda, H. Kariwa, N. Isoda

An outbreak of novel coronavirus infection occurred in China at the end of 2019, which was designated as coronavirus disease 2019 (COVID-19), and spread to regions across Asia and ultimately all over the world As of 21 May 2020, a total of more than 5 million cases with more than 350 thousand deaths were reported worldwide Evaluation of the pathogenicity of the disease and determining the efficacy of control measures are essential for rapid containment of the disease However, the world is facing difficulties in controlling COVID-19 at both of the national and global levels due to variations in pathogenicity of infection by severe acute respiratory syndrome coronavirus 2, the causal agent of COVID-19, and to diverse measures applied in each country based on their control capacities and policies In the present review, we summarize the basic information and findings related to the COVID- 19 pandemic, including pathogen agent, epidemiology, disease transmission, and clinical manifestations Diagnosis, treatment, and preventive measures applied or under development all over the world are also reviewed to provide the opportunity to establish a more effective scenario for disease containment Humanity has progressed by developing countless great technologies and immense scientific theories, however it may be a fact that we cannot conquer all risks to humanity New findings and challenges for the unprecedented pandemic at the global level, such as COVID-19, should also contribute to preparedness for unknown diseases in future, similar to the lessons learnt from severe acute respiratory syndrome and the pandemic A(H1N1)pdm09 influenza

2019年底，中国爆发了一场新型冠状病毒感染疫情，被确定为2019冠状病毒病(COVID-19)，并蔓延至亚洲各地区，最终蔓延至世界各地。截至2020年5月21日，全球累计报告病例超过500万例，死亡人数超过35万人。由于引起COVID-19的病原体冠状病毒2 (sars)感染的致病性存在差异，各国根据控制能力和政策采取的措施也不尽相同，因此在国家和全球范围内控制COVID-19都面临困难。本文综述了COVID-19大流行的基本信息和发现，包括病原体、流行病学、疾病传播、并对世界各地已应用或正在开发的诊断、治疗和预防措施进行审查，为建立更有效的疾病控制方案提供机会。人类通过开发无数伟大的技术和庞大的科学理论取得了进步，但我们可能无法克服人类面临的所有风险，这可能是一个事实。还应促进今后对未知疾病的防范，类似于从严重急性呼吸系统综合症和甲型H1N1流感大流行中吸取的教训

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引用次数: 1

Discovery of genome of an immunodeficiency-associated virus-like virus from pig feces in Japan 从日本猪粪便中发现免疫缺陷相关病毒样病毒的基因组

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2018-01-01 DOI: 10.14943/JJVR.66.1.53

M. Oba, Y. Katayama, S. Tsuchiaka, T. Omatsu, Yoshiteru Murata, K. Ohya, S. Makino, M. Nagai, T. Mizutani

Immunodeficiency-associated stool virus (IASV) is an unclassified virus, for which the only genome information is available from a patient infected by human immunodeficiency virus. In domestic animals, one report described detection of IASV-like virus in pig feces, whereas no nucleotide sequence information of this virus is currently available. Using deep sequencing method, we detected a DNA fragment homologous to IASV in several pig feces in Japan. The sequence of the PCR product in this sample had 70% homology to that of IASV. The infectious rate of the IASV-like virus was 72.9% among the 9 pig farms, from which the samples were collected. There was no clear correlation between the presence of IASV-like virus and the fecal characteristics.

免疫缺陷相关粪便病毒(IASV)是一种未分类的病毒，其唯一的基因组信息来自感染人类免疫缺陷病毒的患者。在家畜中，一份报告描述了在猪粪便中检测到iasv样病毒，但目前尚无该病毒的核苷酸序列信息。采用深度测序方法，在日本猪粪便中检测到与IASV同源的DNA片段。本样本PCR产物序列与IASV同源性达70%。采集样本的9个猪场的iasv样病毒感染率为72.9%。iasv样病毒的存在与粪便特征无明显相关性。

引用次数: 2

Signal changes in standing magnetic resonance imaging of septic pedal osteitis in a thoroughbred racehorse 纯种赛马脓毒性足部骨炎的站立磁共振成像信号变化

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2017-05-01 DOI: 10.14943/JJVR.65.2.101

Fumiaki Mizobe, M. Nomura, Tomohiro Kato, Yoshinari Katayama, A. Kuwano, T. Ueno, Kazutaka Yamada, M. Spriet

Septic pedal osteitis refers to bacterial infection within the distal phalanx, which requires effective surgical intervention. A 3-year-old Thoroughbred colt underwent standing magnetic resonance imaging (sMRI) on the 10th day after admission (day 10), which revealed hyperintensity beneath the inner hoof wall in sagittal T2*-weighted and transverse T2-weighted sequence, suggesting abscess formation. On day 25, the second sMRI indicated development of osteitis, in which the lesion extended into the distal phalanx. This lesion was imaged as hyperintensity on T1-weighted, T2*-weighted and T2-weighted images. On day 59, laminitic changes were evident on T1-weighted and T2*-weighted images. Our case report suggests that sMRI could allow better understanding of the disease process than other conventional imaging modalities, and guide early surgical intervention.

脓毒症足骨炎是指远端指骨内的细菌感染，需要有效的手术干预。一匹3岁的纯种小马在入院后第10天（第10天）接受了站立磁共振成像（sMRI）检查，结果显示，在矢状T2*加权和横向T2加权序列中，内蹄壁下方出现高信号，提示脓肿形成。第25天，第二次sMRI显示出现骨炎，病变延伸至远端指骨。该病变在T1加权、T2*加权和T2加权图像上被成像为高信号。第59天，在T1加权和T2*加权图像上，层粘连明显改变。我们的病例报告表明，与其他传统成像方式相比，sMRI可以更好地了解疾病过程，并指导早期手术干预。

引用次数: 0

Identification of novel alternative splicing variants within swine Setd8 gene and their high mRNA expression in testis. 猪Setd8基因新的选择性剪接变异的鉴定及其在睾丸中的高mRNA表达。

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2017-02-01

Shuai Yu, Xiaoxu Chen, Zhongyuan Deng, Xianyong Lan, Chuanying Pan

SET domain containing (lysine methyltransferase) 8 (Setd8), a histone modification enzyme, affects cell cycling, chromosome condensation, high efficient repair of DNA double strand breaks and so on. The objective of this study was to identify novel alternative splicing variants of pig Setd8 gene and its mRNA expression. Four 180-day-old male Guanzhong Black (GZB) pigs and six male Landrace piglets (including three 30-day-old and three 7-day-old pigs) were collected to study Setd8 gene. Herein, two novel variants, Setd8a and Setd8b, were found in pig. The entire sequences of Setd8a and Setd8b variants were 1,039 bp and 958 bp, respectively. qRT-PCR results showed that Setd8a and Setd8b were highly expressed in brains and testes of 180-day-old GZB pigs. Moreover, the expressions of the two Setd8 variants were significantly higher in testis than brain of GZB pig (P < 0.05). Further study on testis showed that the mRNA expression of Setd8a variant was significantly lower than Setd8b variant in 30-day-old and 7-day-old pigs (P < 0.05). The mRNA expression of Setd8a variant'was lower than Setd8b variant in GZB pigs (P > 0.05). Moreover, the expressions of the two Setd8 variants were significantly higher along with age enlargement. In conclusion, Setd8a and Setd8b were firstly identified in pigs and both were expressed in pig testis. Setd8b was the major splicing variant of pig Seid8 gene transcript product. Moreover, the expressions of Setd8 variants were time-dependent. All these findings would enrich the study of Setd8 gene in pig testis.

SET结构域含有组蛋白修饰酶(赖氨酸甲基转移酶)8 (Setd8)，影响细胞循环、染色体凝聚、DNA双链断裂的高效修复等。本研究的目的是鉴定猪Setd8基因的新选择性剪接变体及其mRNA表达。收集4头180日龄关中黑猪(GZB)和6头长白猪(3头30日龄和3头7日龄)进行Setd8基因研究。本文在猪中发现了Setd8a和Setd8b两个新的变异体。Setd8a和Setd8b变体的全序列分别为1039 bp和958 bp。qRT-PCR结果显示，Setd8a和Setd8b在180日龄GZB猪的大脑和睾丸中高表达。此外，两种Setd8基因在GZB猪睾丸中的表达量显著高于脑(P < 0.05)。对30日龄和7日龄猪睾丸的进一步研究表明，Setd8a变异的mRNA表达量显著低于Setd8b变异(P < 0.05)。GZB猪中Setd8a基因的mRNA表达量低于Setd8b基因的表达量(P > 0.05)。此外，随着年龄的增大，两种Setd8变异的表达量显著升高。综上所述，Setd8a和Setd8b基因首次在猪中被发现，且均在猪睾丸中表达。Setd8b是猪Seid8基因转录产物的主要剪接变体。此外，Setd8变体的表达具有时间依赖性。这些发现将丰富猪睾丸Setd8基因的研究。

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引用次数: 0

Anti-neoplastic effects of topoisomerase inhibitors in canine mammary carcinoma, melanoma, and osteosarcoma cell lines. 拓扑异构酶抑制剂在犬乳腺癌、黑色素瘤和骨肉瘤细胞系中的抗肿瘤作用。

IF 0.4 4区农林科学 Q3 Veterinary

Japanese Journal of Veterinary Research

Pub Date : 2017-02-01

Siew Mei Ong, Hiroki Yamamoto, Kohei Saeki, Yuiko Tanaka, Ryohei Yoshitake, Ryohei Nishimura, Takayuki Nakagawa

Numerous topoisomerase inhibitors with proven efficacy have been used extensively to treat various human neoplasms. However, among these, only doxorubicin has been used and studied extensively in veterinary oncology. The current study was performed to evaluate the responsiveness of canine osteosarcoma (cOSA), mammary gland tumour (cMGT), and malignant melanoma (cMM) cell lines to several topoisomerase inhibitors. In addition, the correlation between the sensitivity to treatment and multi-drug resistant (MDR) factors was investigated. cOSA cell lines exhibited higher sensitivity than cMGT and cMM cell lines to all the topoisomerase inhibitors tested in vitro; this was associated with the levels of multi-drug resistance protein 1 (MDR1) gene expression in the cOSA cell lines. Treatment of cOSA (HMPOS) and cMGT cell line (CHMp) xenograft mouse models with etoposide markedly delayed tumour progression in HMPOS xenografts, but failed to elicit lasting anti-tumour effects on CHMp xenograft mice. The present findings suggest that MDR1 represents a molecular signature for prediction of treatment efficacy of topoisomerase inhibitors, especially that of etoposide, which may be a clinically useful anti-tumour agent for cOSA; however, further study is necessary to refine the treatment protocol.

许多拓扑异构酶抑制剂已被证实有效，广泛用于治疗各种人类肿瘤。然而，其中只有阿霉素在兽医肿瘤学中得到了广泛的应用和研究。目前的研究是为了评估犬骨肉瘤(cOSA)、乳腺肿瘤(cMGT)和恶性黑色素瘤(cMM)细胞系对几种拓扑异构酶抑制剂的反应性。此外，还探讨了多药耐药(MDR)因素与治疗敏感性的关系。cOSA细胞株对所有拓扑异构酶抑制剂的敏感性均高于cMGT和cMM细胞株;这与cOSA细胞系中多药耐药蛋白1 (MDR1)基因表达水平有关。用依托泊苷治疗cOSA (HMPOS)和cMGT细胞系(CHMp)异种移植小鼠模型，显著延缓了HMPOS异种移植小鼠的肿瘤进展，但未能对CHMp异种移植小鼠产生持久的抗肿瘤作用。目前的研究结果表明，MDR1代表了预测拓扑异构酶抑制剂治疗效果的分子特征，特别是依托泊苷，它可能是临床有用的cOSA抗肿瘤药物;然而，需要进一步的研究来完善治疗方案。

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