Production of Monoclonal Antibodies
Wayne M. Yokoyama, Michelle Christensen, Gary Dos Santos, Diane Miller, Jason Ho, Tao Wu, Michael Dziegelewski, Francisca A. Neethling
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引用次数: 38
Abstract
This unit describes the production of monoclonal antibodies beginning with immunization, cell fusion, and selection. Support protocols are provided for screening primary hybridoma supernatants for antibodies of desired specificity, establishment of stable hybridoma lines, cloning of these B cell lines by limiting dilution to obtain monoclonal lines, and preparation of cloning/expansion medium. An alternate protocol describes cell fusion and one-step selection and cloning of hybridomas utilizing a semi-solid methylcellulose-based medium (ClonaCell-HY from StemCell Technologies). Curr. Protoc. Immunol. 102:2.5.1-2.5.29. © 2013 by John Wiley & Sons, Inc.
单克隆抗体的生产
本单元描述了从免疫、细胞融合和选择开始的单克隆抗体的生产。提供了筛选原代杂交瘤上清液中所需特异性抗体的支持方案，建立稳定的杂交瘤系，通过限制稀释获得单克隆系克隆这些B细胞系，并制备克隆/扩增培养基。另一种方案描述了利用半固体甲基纤维素为基础的培养基(来自StemCell Technologies的ClonaCell-HY)进行细胞融合和一步选择和克隆杂交瘤。咕咕叫。Protoc。Immunol 102:2.5.1-2.5.29。©2013 by John Wiley &儿子,Inc。
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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