Issue Information

Abstract

Cover: In Vanderheiden et al. (http://doi.org/10.1002/cpim.116), the image shows overview of high-throughput FRNT assay for measuring SARS CoV-2 neutralizing antibodies. Flow chart demonstrating the experimental outline. In brief, the specimens are serially diluted and mixed with equal parts of icSARS-CoV-2 following 1 hr incubation at 37^oC with 5% CO₂. Then, the immune complex mixture is overlaid on top of Vero E6 cells and incubated at 37^oC and 5% CO₂ for 1 hr. The viral inoculum is removed and replaced by 0.85% methylcellulose. Infected cultures are incubated for 24 hr at 37^oC with 5% CO₂. After this, the cells are fixed with paraformaldehyde and probed for SARS-CoV-2 spike protein. Foci are visualized via chromogen deposit, recorded using CTL ImmunoSpot S6 Universal Analyzer, and quantified using Viridot.