Hypercholesterolemic diet induces hepatic steatosis and alterations in mRNA expression of NADPH oxidase in rat livers.

Arquivos brasileiros de endocrinologia e metabologia Pub Date : 2014-04-01 DOI:10.1590/0004-2730000002831

Isabel Cristina Mallosto Emerich de Abreu, Joyce Ferreira da Costa Guerra, Renata Rebeca Pereira, Maísa Silva, Wanderson Geraldo de Lima, Marcelo Eustáquio Silva, Maria Lúcia Pedrosa

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引用次数: 29

Abstract

Objective: This study aimed to determine whether a hypercholesterolemic diet induces hepatic steatosis, alterations in mRNA expression of NADPH oxidase subunits, and antioxidant defenses.

Materials and methods: Fischer rats were divided into two groups of eight animals according to the treatment, control (C) and hypercholesterolemic diet (H). Those in group C were fed a standard diet (AIN-93M), and those of the group H were fed a hypercholesterolemic diet (25% soybean oil and 1% cholesterol).

Results: The hypercholesterolemic diet did not affect body weight, but resulted in the accumulation of lipids in the liver, increased serum activities of aminotransferases and cholesterol levels. Biomarker of lipid peroxidation (TBARS) and mRNA expression of NADPH oxidase subunits p22(phox) and p47(phox) were increased in the liver of animals in group H. Besides, the activity and expression of antioxidant enzymes were altered.

Conclusion: The results show increased mRNA expression of NADPH oxidase subunits and changes in antioxidant enzyme activities in diet-induced hepatic steatosis.

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高胆固醇饮食诱导大鼠肝脏脂肪变性和NADPH氧化酶mRNA表达的改变。

目的:本研究旨在确定高胆固醇饮食是否会导致肝脂肪变性、NADPH氧化酶亚基mRNA表达的改变和抗氧化防御。材料与方法:将Fischer大鼠按对照组(C)和高胆固醇血症饲粮(H)分为两组，每组8只，C组饲喂标准饲粮(AIN-93M)， H组饲喂高胆固醇血症饲粮(25%大豆油+ 1%胆固醇)。结果:高胆固醇饮食不影响体重，但导致肝脏脂质堆积，血清转氨酶活性和胆固醇水平升高。h组动物肝脏脂质过氧化生物标志物(TBARS)和NADPH氧化酶亚基p22(phox)、p47(phox) mRNA表达量升高，抗氧化酶活性和表达发生改变。结论:饮食诱导的肝脂肪变性中NADPH氧化酶亚基mRNA表达增加，抗氧化酶活性发生变化。

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