Positive effects of naringenin on near-surface membrane fluidity in human erythrocytes.

Acta physiologica Hungarica Pub Date : 2015-06-01 DOI:10.1556/036.102.2015.2.3

Vladimir Ajdžanović, V Jakovljević, D Milenković, A Konić-Ristić, J Živanović, I Jarić, V Milošević

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引用次数: 8

Abstract

Purpose: Deformability/rheologic behavior of erythrocytes are related to near-surface membrane fluidity. Specific agents can increase erythrocyte membrane fluidity in order to adjust hemodynamics in cardiovascular diseases. Grapefruit flavanone naringenin has been proposed for potential use in an alternative therapy of cardiovascular conditions. In respect to this, we assessed here effects of two nutritionally relevant concentrations of naringenin (0.1 and 1 μg/ml) on near-surface membrane fluidity in human erythrocytes.

Methods: We used electron paramagnetic resonance spectroscopy and fatty acid spin probes (5-DS and 7-DS), the spectra of which are dependent on membrane fluidity.

Results: The results showed a significant (p = 0.029) increase of membrane fluidity near the hydrophilic surface in erythrocytes treated with higher concentration of naringenin. In the deeper layer, just below the erythrocyte membrane phospholipid heads, both lower and higher concentration of naringenin significantly increased membrane fluidity (p = 0.036 and p = 0.028, respectively).

Conclusions: These data document the positive and dose dependent effect of naringenin on near-surface membrane fluidity in human erythrocytes, recommending its use in the cardiovascular conditions characterized by disturbed hemodynamics.

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柚皮素对人红细胞近表面膜流动性的积极影响。

目的:红细胞的变形性/流变学行为与近表面膜流动性有关。特定药物可增加红细胞膜流动性，以调节心血管疾病的血流动力学。葡萄柚黄酮柚皮素已被提议用于潜在的替代治疗心血管疾病。为此，我们评估了两种营养相关浓度的柚皮素(0.1和1 μg/ml)对人红细胞近表面膜流动性的影响。方法:采用电子顺磁共振波谱法和脂肪酸自旋探针(5-DS和7-DS)，它们的光谱依赖于膜的流动性。结果:高浓度柚皮素使红细胞亲水表面附近的膜流动性显著增加(p = 0.029)。在红细胞膜磷脂头下方的较深层，柚皮素浓度较低和较高均显著增加了细胞膜流动性(p = 0.036和p = 0.028)。结论:这些数据证明柚皮素对人红细胞近表面膜流动性的正向和剂量依赖性作用，推荐其用于以血流动力学紊乱为特征的心血管疾病。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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Acta physiologica Hungarica 医学-生理学

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6.0 months