Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.2
S. Nadjafi, S. Ebrahimi, N. Rahbar‐Roshandel
This study was carried out to evaluate the effects of noscapine, a benzylisoquinoline alkaloid from opium poppy, on oligodendrocyte during ischemia/reperfusion-induced excitotoxic injury. Changes in intracellular calcium levels due to chemical ischemia and nitric oxide (NO) production during ischemia/reperfusion were evaluated as the hallmarks of ischemia-derived excitotoxic event. OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. 30- or 60-minute-oxygen-glucose deprivation/24 hours reperfusion were used to induce excitotoxicity. MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay was used to evaluate cell viability. Ratiometric fluorescence microscopy using Ca(2+)-sensitive indicator Fura-2/AM was utilized to assess intracellular calcium levels. NO production was evaluated by Griess method. Noscapine (4 μM) significantly attenuated intracellular Ca(2+) elevation (P < 0.001). Also, noscapine significantly decreased NO production during a 30-minute oxygen-glucose deprivation/reperfusion (P < 0.01). The inhibitory effect of noscapine (4 μM) on intracellular Ca(2+) was greater than ionotropic glutamate receptors antagonists. Noscapine is protective against ischemia/reperfusion-induced excitotoxic injury in OLN-93 oligodendrocyte. This protective effect seems to be related to attenuation of intracellular Ca(2+) overload and NO production.
{"title":"Noscapine protects OLN-93 oligodendrocytes from ischemia-reperfusion damage: Calcium and nitric oxide involvement.","authors":"S. Nadjafi, S. Ebrahimi, N. Rahbar‐Roshandel","doi":"10.1556/036.102.2015.4.2","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.2","url":null,"abstract":"This study was carried out to evaluate the effects of noscapine, a benzylisoquinoline alkaloid from opium poppy, on oligodendrocyte during ischemia/reperfusion-induced excitotoxic injury. Changes in intracellular calcium levels due to chemical ischemia and nitric oxide (NO) production during ischemia/reperfusion were evaluated as the hallmarks of ischemia-derived excitotoxic event. OLN-93 cell line (a permanent immature rat oligodendrocyte) was used as a model of oligodendrocyte. 30- or 60-minute-oxygen-glucose deprivation/24 hours reperfusion were used to induce excitotoxicity. MTT (3-[4,5-Dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide) assay was used to evaluate cell viability. Ratiometric fluorescence microscopy using Ca(2+)-sensitive indicator Fura-2/AM was utilized to assess intracellular calcium levels. NO production was evaluated by Griess method. Noscapine (4 μM) significantly attenuated intracellular Ca(2+) elevation (P < 0.001). Also, noscapine significantly decreased NO production during a 30-minute oxygen-glucose deprivation/reperfusion (P < 0.01). The inhibitory effect of noscapine (4 μM) on intracellular Ca(2+) was greater than ionotropic glutamate receptors antagonists. Noscapine is protective against ischemia/reperfusion-induced excitotoxic injury in OLN-93 oligodendrocyte. This protective effect seems to be related to attenuation of intracellular Ca(2+) overload and NO production.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"351-62"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67628096","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.9
F. Köteles, Z. Dömötör, T. Berkes, R. Szemerszky
Polar watches with heart rate monitoring function have become popular among recreational and professional athletes. In addition to monitoring functions, they calculate a specific index called OwnIndex which is claimed to measure aerobic training status. The current research attempted to shed light on the factors determining the OwnIndex. In Study 1, OwnIndex calculated by the RS-400 Polar watch was estimated using anthropometric (gender, age, height, weight), cardiovascular (resting HR, RMSSD), and exercise-related (maximal oxygen uptake, self-reported physical activity) data of 45 young adults. In Study 2, the OwnIndex was measured in 21 young adults twice, first with self-reported physical activity set to the lowest, then to the highest value. In the regression analysis (Study 1), the only significant predictor of OwnIndex was self-reported physical activity (R² = 0.883; β = 0.915, p < 0.001). A significant difference with a large effect size (t(20) = -16.657, p <0.001, d = 3.635) and no significant correlation (r = -0.32; p = 0.155) were found between the OwnIndices calculated with different levels of activity in Study 2. As anthropometric and cardiac variables play a practically negligible role in the calculation of the OwnIndex, it cannot be considered an appropriate measure of aerobic fitness.
具有心率监测功能的极地手表在休闲和专业运动员中很受欢迎。除了监测功能外，他们还计算了一个名为OwnIndex的特定指数，据称该指数可以衡量有氧训练的状态。目前的研究试图揭示决定OwnIndex的因素。在研究1中，利用45名年轻人的人体测量(性别、年龄、身高、体重)、心血管(静息心率、RMSSD)和运动相关(最大摄氧量、自我报告的身体活动)数据，通过RS-400 Polar手表计算出OwnIndex。在研究2中，对21名年轻人进行了两次OwnIndex测量，首先将自我报告的体力活动设置为最低值，然后设置为最高值。在回归分析(研究1)中，OwnIndex的唯一显著预测因子是自我报告的体育活动(R²= 0.883;β = 0.915, p < 0.001)。差异显著且效应量大(t(20) = -16.657, p <0.001, d = 3.635)，无显著相关性(r = -0.32;p = 0.155)，在研究2中不同运动水平计算的OwnIndices之间存在差异。由于人体测量和心脏变量在OwnIndex的计算中所起的作用几乎可以忽略不计，因此它不能被认为是一种适当的有氧健身指标。
{"title":"Polar OwnIndex is not a reliable indicator of aerobic training status.","authors":"F. Köteles, Z. Dömötör, T. Berkes, R. Szemerszky","doi":"10.1556/036.102.2015.4.9","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.9","url":null,"abstract":"Polar watches with heart rate monitoring function have become popular among recreational and professional athletes. In addition to monitoring functions, they calculate a specific index called OwnIndex which is claimed to measure aerobic training status. The current research attempted to shed light on the factors determining the OwnIndex. In Study 1, OwnIndex calculated by the RS-400 Polar watch was estimated using anthropometric (gender, age, height, weight), cardiovascular (resting HR, RMSSD), and exercise-related (maximal oxygen uptake, self-reported physical activity) data of 45 young adults. In Study 2, the OwnIndex was measured in 21 young adults twice, first with self-reported physical activity set to the lowest, then to the highest value. In the regression analysis (Study 1), the only significant predictor of OwnIndex was self-reported physical activity (R² = 0.883; β = 0.915, p < 0.001). A significant difference with a large effect size (t(20) = -16.657, p <0.001, d = 3.635) and no significant correlation (r = -0.32; p = 0.155) were found between the OwnIndices calculated with different levels of activity in Study 2. As anthropometric and cardiac variables play a practically negligible role in the calculation of the OwnIndex, it cannot be considered an appropriate measure of aerobic fitness.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"419-27"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67628299","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.4
S. Shekarforoush, F. Safari
Some researchers have reported that lactation is effective in reducing cardiovascular disease risk factors. The purpose of this study was to investigate whether lactation may improve intrinsic tolerance against ischemia reperfusion (IR) injury. The rats were randomly divided into two groups (n = 8 in each group). In the lactation (Lact) group, the surgery was performed on postpartum day 21 (at the end of lactation period) and the results were compared with those of virgin female rats (control group). Cardiac IR injury was induced by means of left anterior descending coronary artery occlusion for 30 min followed by reperfusion for 120 min. Infarct size was measured using the staining agent 2,3,5-triphenyltetrazolium chloride. At the end of the experiment, Mean arterial pressure in the control group was significantly lower than that in the Lact group. Myocardial infarct size was significantly reduced in the Lact group (23 ± 3% vs. 45 ± 8%, p < 0.05 in the control group). Lactation reduced the extent of myocardial injury induced by ischemia and reperfusion. So, lactation may increase cardiac tolerance to ischemic injury.
一些研究人员报道，哺乳对减少心血管疾病的危险因素是有效的。本研究的目的是探讨哺乳是否可以提高对缺血再灌注(IR)损伤的内在耐受性。将大鼠随机分为两组，每组8只。哺乳期(Lact)组于产后第21天(哺乳期结束)进行手术，并与未交配雌性大鼠(对照组)进行比较。采用左冠状动脉前降支闭塞30 min，再灌注120 min的方法诱导心肌IR损伤。用染色剂2,3,5-三苯四唑氯测定梗死面积。实验结束时，对照组的平均动脉压显著低于Lact组。Lact组心肌梗死面积明显减小(23±3% vs. 45±8%，对照组p < 0.05)。哺乳可减轻心肌缺血再灌注损伤程度。因此，哺乳可能增加心脏对缺血性损伤的耐受性。
{"title":"Lactation protects against myocardial ischemia-reperfusion injury in rats.","authors":"S. Shekarforoush, F. Safari","doi":"10.1556/036.102.2015.4.4","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.4","url":null,"abstract":"Some researchers have reported that lactation is effective in reducing cardiovascular disease risk factors. The purpose of this study was to investigate whether lactation may improve intrinsic tolerance against ischemia reperfusion (IR) injury. The rats were randomly divided into two groups (n = 8 in each group). In the lactation (Lact) group, the surgery was performed on postpartum day 21 (at the end of lactation period) and the results were compared with those of virgin female rats (control group). Cardiac IR injury was induced by means of left anterior descending coronary artery occlusion for 30 min followed by reperfusion for 120 min. Infarct size was measured using the staining agent 2,3,5-triphenyltetrazolium chloride. At the end of the experiment, Mean arterial pressure in the control group was significantly lower than that in the Lact group. Myocardial infarct size was significantly reduced in the Lact group (23 ± 3% vs. 45 ± 8%, p < 0.05 in the control group). Lactation reduced the extent of myocardial injury induced by ischemia and reperfusion. So, lactation may increase cardiac tolerance to ischemic injury.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"372-9"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627794","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.12
E. Bosnyák, E. Trájer, A. Udvardy, Z. Komka, A. Protzner, T. Kováts, I. Györe, M. Tóth, J. Pucsok, M. Szmodis
The aim of the study was to determine the importance of two sport-associated gene polymorphisms, alpha-actinin-3 R577X (ACTN3) and angiotensin-converting enzyme I/D (ACE), among Hungarian athletes in different sports. The examination was carried out only on women (n = 100). Sport-specific groups were formed in order to guarantee the most homogeneous clusters. Human genomic DNA was isolated from blood, and genotyping was performed by polymerase chain reaction. To measure the differences between the participating groups, Chi-squared test was performed using Statistica 9.0 for Windows® (significance level: p < 0.05). In comparing the ACE I/D allele frequencies, significant difference was detected between water polo (I = 61.11%; D = 38.89%) and combat sports (I = 35.71%, D = 64.29%) athletes (p < 0.03). There was no statistical difference when ACE I/D alleles in combat sports and kayaking/rowing (p > 0.05) were compared. A similarity was detectable in the I allele frequencies of the water polo (61.11%) and kayaking/rowing (56.67%) groups. The ACTN3 R/X polymorphism showed no differences in comparison with the sport groups. R allele frequencies were higher in every group compared to the X allele. The potential significance of the ACE I allele in sports of an aerobic nature was not clearly confirmed among Hungarian athletes.
{"title":"ACE and ACTN3 genes polymorphisms among female Hungarian athletes in the aspect of sport disciplines.","authors":"E. Bosnyák, E. Trájer, A. Udvardy, Z. Komka, A. Protzner, T. Kováts, I. Györe, M. Tóth, J. Pucsok, M. Szmodis","doi":"10.1556/036.102.2015.4.12","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.12","url":null,"abstract":"The aim of the study was to determine the importance of two sport-associated gene polymorphisms, alpha-actinin-3 R577X (ACTN3) and angiotensin-converting enzyme I/D (ACE), among Hungarian athletes in different sports. The examination was carried out only on women (n = 100). Sport-specific groups were formed in order to guarantee the most homogeneous clusters. Human genomic DNA was isolated from blood, and genotyping was performed by polymerase chain reaction. To measure the differences between the participating groups, Chi-squared test was performed using Statistica 9.0 for Windows® (significance level: p < 0.05). In comparing the ACE I/D allele frequencies, significant difference was detected between water polo (I = 61.11%; D = 38.89%) and combat sports (I = 35.71%, D = 64.29%) athletes (p < 0.03). There was no statistical difference when ACE I/D alleles in combat sports and kayaking/rowing (p > 0.05) were compared. A similarity was detectable in the I allele frequencies of the water polo (61.11%) and kayaking/rowing (56.67%) groups. The ACTN3 R/X polymorphism showed no differences in comparison with the sport groups. R allele frequencies were higher in every group compared to the X allele. The potential significance of the ACE I allele in sports of an aerobic nature was not clearly confirmed among Hungarian athletes.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"451-8"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.12","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.13
Y.-M. Song, H. Hsu, M.-D. Chen
Zinc (Zn) has the potential of regulating the action of thiazolidinedione (TZD), an anti-diabetic drug. Since some diabetic patients cannot achieve optimal glycemic control when receiving TZD, we investigated if Zn deficiency affects TZD's efficacy in glucose metabolism. Diabetic mice were fed diets containing 3 or 30 mg/kg Zn for 6 weeks. Thereafter, all mice were oral gavaged with 2,4-thiazolidinedione. Our results showed that blood glucose values at fasting and during the glucose tolerance test were significantly higher in low-Zn mice than those of adequate-Zn mice. Thus, low Zn intake may attenuate TZD's efficacy on reducing diabetic hyperglycemia.
{"title":"Low dietary zinc intake attenuates the efficacy of 2,4-thiazolidinedione on reducing hyperglycemia in db/db mice (Short communication).","authors":"Y.-M. Song, H. Hsu, M.-D. Chen","doi":"10.1556/036.102.2015.4.13","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.13","url":null,"abstract":"Zinc (Zn) has the potential of regulating the action of thiazolidinedione (TZD), an anti-diabetic drug. Since some diabetic patients cannot achieve optimal glycemic control when receiving TZD, we investigated if Zn deficiency affects TZD's efficacy in glucose metabolism. Diabetic mice were fed diets containing 3 or 30 mg/kg Zn for 6 weeks. Thereafter, all mice were oral gavaged with 2,4-thiazolidinedione. Our results showed that blood glucose values at fasting and during the glucose tolerance test were significantly higher in low-Zn mice than those of adequate-Zn mice. Thus, low Zn intake may attenuate TZD's efficacy on reducing diabetic hyperglycemia.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"459-63"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.13","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67628011","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.7
D. Gradinaru, D. Margină, M. Ilie, C. Borșa, C. Ionescu, G. Prada
Erythropoietin (EPO), a key hormone involved in red blood cell formation has been recently acknowledged for its pleiotropic actions and protective role in ageing and various pathological conditions concurrent with oxidative stress, vascular diseases and metabolic abnormalities such as diabetes mellitus. The aim of the study was to evaluate the relationship between circulating erythropoietin levels and oxidative stress biomarkers, in elderly with type 2 diabetes (T2DM). The study was carried out in 67 subjects with T2DM (69 ± 5 years; n = 37) without anemia, and aged-matched controls (70 ± 6 years; n = 30). EPO serum levels, erythrocyte susceptibility to lipid peroxidation (ESP) and total antioxidant capacity (TAC) were evaluated. Lower EPO levels (p < 0.01) and higher ESP values (p < 0.001) were found in T2DM group, compared to healthy subjects. EPO levels showed significant negative associations with ESP, both in T2DM subjects (r = -0.565; p < 0.001) and in all study population (r = -0,600; p < 0,001; n = 67). In conclusion, we provide new data regarding the cytoprotective effect of EPO exerted at systemic level on erythrocyte membrane, in the particular state of impaired glucose metabolism associated with oxidative stress, in the elderly.
{"title":"Correlation between erythropoietin serum levels and erythrocyte susceptibility to lipid peroxidation in elderly with type 2 diabetes.","authors":"D. Gradinaru, D. Margină, M. Ilie, C. Borșa, C. Ionescu, G. Prada","doi":"10.1556/036.102.2015.4.7","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.7","url":null,"abstract":"Erythropoietin (EPO), a key hormone involved in red blood cell formation has been recently acknowledged for its pleiotropic actions and protective role in ageing and various pathological conditions concurrent with oxidative stress, vascular diseases and metabolic abnormalities such as diabetes mellitus. The aim of the study was to evaluate the relationship between circulating erythropoietin levels and oxidative stress biomarkers, in elderly with type 2 diabetes (T2DM). The study was carried out in 67 subjects with T2DM (69 ± 5 years; n = 37) without anemia, and aged-matched controls (70 ± 6 years; n = 30). EPO serum levels, erythrocyte susceptibility to lipid peroxidation (ESP) and total antioxidant capacity (TAC) were evaluated. Lower EPO levels (p < 0.01) and higher ESP values (p < 0.001) were found in T2DM group, compared to healthy subjects. EPO levels showed significant negative associations with ESP, both in T2DM subjects (r = -0.565; p < 0.001) and in all study population (r = -0,600; p < 0,001; n = 67). In conclusion, we provide new data regarding the cytoprotective effect of EPO exerted at systemic level on erythrocyte membrane, in the particular state of impaired glucose metabolism associated with oxidative stress, in the elderly.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"400-8"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67628430","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.3
A. Szabó, I. Bocșan, Ş. Suciu, A. Buzoianu
UNLABELLED Pain relief using drugs with high efficacy provides significant improvement in the patients' lives. Drugs like lamotrigine (LTG) and gabapentin (GBP) have the ability to overcome the symptoms of neuropathic pain. AIM The present study offers a comparative analysis of LTG and GBP efficacy in a rat model of nociceptive pain after single administration. METHOD Sixty-three Wistar-Bratislava rats randomized into 7 groups were included: a control group treated with saline solution and 6 groups treated with different doses of LTG and GBP. Nociceptive responses to thermal and mechanical stimulations were evaluated before and after drug administration, at different time intervals, using paw pressure and hot plate tests. The obtained data were statistically analyzed, with significance at p value < 0.05. RESULTS LTG 100 mg/kg and 50 mg/kg presented a significant analgesic effect in both mechanical and thermal tests, 1 and 2 hours after administration. GBP 100 mg/kg increased latency time in hot plate test. The effect of both anticonvulsant drugs occurred rapidly after administration, but had a short duration. CONCLUSIONS LTG and GBP had an analgesic effect in a single dose administration. The effect of LTG was more evident since it was observed in both tests. Their effect was dose dependent.
{"title":"Comparative animal study of the antinociceptive efficacy of lamotrigine and gabapentin for the management of pain.","authors":"A. Szabó, I. Bocșan, Ş. Suciu, A. Buzoianu","doi":"10.1556/036.102.2015.4.3","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.3","url":null,"abstract":"UNLABELLED\u0000Pain relief using drugs with high efficacy provides significant improvement in the patients' lives. Drugs like lamotrigine (LTG) and gabapentin (GBP) have the ability to overcome the symptoms of neuropathic pain.\u0000\u0000\u0000AIM\u0000The present study offers a comparative analysis of LTG and GBP efficacy in a rat model of nociceptive pain after single administration.\u0000\u0000\u0000METHOD\u0000Sixty-three Wistar-Bratislava rats randomized into 7 groups were included: a control group treated with saline solution and 6 groups treated with different doses of LTG and GBP. Nociceptive responses to thermal and mechanical stimulations were evaluated before and after drug administration, at different time intervals, using paw pressure and hot plate tests. The obtained data were statistically analyzed, with significance at p value < 0.05.\u0000\u0000\u0000RESULTS\u0000LTG 100 mg/kg and 50 mg/kg presented a significant analgesic effect in both mechanical and thermal tests, 1 and 2 hours after administration. GBP 100 mg/kg increased latency time in hot plate test. The effect of both anticonvulsant drugs occurred rapidly after administration, but had a short duration.\u0000\u0000\u0000CONCLUSIONS\u0000LTG and GBP had an analgesic effect in a single dose administration. The effect of LTG was more evident since it was observed in both tests. Their effect was dose dependent.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"363-71"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.3","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.10
D. Kim, J. Loenneke, R. Thiebaud, T. Abe, M. Bemben
The aim was to compare the acute effects of work matched high intensity (75% peak aerobic capacity) aerobic exercise to low intensity (40% peak aerobic capacity) aerobic exercise with different degrees of blood flow restriction (BFR) [40% estimated arterial occlusion (40 BFR) and 60% estimated arterial occlusion (60 BFR)] on variables previously hypothesized to be important for muscle adaptation. There were no meaningful changes in torque. Anterior thigh muscle thickness was increased from baseline with high intensity cycling and 40 BFR (~2 mm increase, p ≤ 0.008). A significant increase in lactate occurred in all exercise conditions but was greatest with high intensity cycling (~5.4 mmol/L increase). Muscle activation was significantly higher with high intensity cycling compared to low intensity cycling with BFR, regardless of pressure (~25% vs. ~12% MVC). Mean power frequency was not different between conditions but did increase from the first 5 minutes of exercise to the last 5 minutes (93% vs. 101%, p < 0.001). Ratings of perceived exertion (RPE) were higher with high intensity cycling but discomfort was similar between conditions. We wish to suggest that high intensity cycling produces greater muscular stress than that observed with work matched low intensity cycling in combination with BFR.
目的是比较工作匹配的高强度(75%峰值有氧能力)有氧运动与低强度(40%峰值有氧能力)有氧运动在不同程度的血流量限制(BFR)[40%估计动脉闭塞(40 BFR)和60%估计动脉闭塞(60 BFR)]对先前假设的对肌肉适应重要变量的急性影响。扭矩没有明显的变化。高强度循环和40 BFR组大腿前肌厚度较基线增加(约2 mm, p≤0.008)。乳酸在所有运动条件下均显著增加，但在高强度循环时增幅最大(约5.4 mmol/L)。无论压力如何，与低强度循环相比，高强度循环的肌肉激活明显更高(~25% vs ~12% MVC)。平均功率频率在不同情况下没有差异，但从运动的前5分钟到最后5分钟确实增加了(93%对101%，p < 0.001)。高强度骑车时，感知运动强度(RPE)评分较高，但两种情况下的不适程度相似。我们希望表明，高强度的循环产生更大的肌肉压力比工作匹配低强度循环结合BFR观察到的。
{"title":"The acute muscular effects of cycling with and without different degrees of blood flow restriction.","authors":"D. Kim, J. Loenneke, R. Thiebaud, T. Abe, M. Bemben","doi":"10.1556/036.102.2015.4.10","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.10","url":null,"abstract":"The aim was to compare the acute effects of work matched high intensity (75% peak aerobic capacity) aerobic exercise to low intensity (40% peak aerobic capacity) aerobic exercise with different degrees of blood flow restriction (BFR) [40% estimated arterial occlusion (40 BFR) and 60% estimated arterial occlusion (60 BFR)] on variables previously hypothesized to be important for muscle adaptation. There were no meaningful changes in torque. Anterior thigh muscle thickness was increased from baseline with high intensity cycling and 40 BFR (~2 mm increase, p ≤ 0.008). A significant increase in lactate occurred in all exercise conditions but was greatest with high intensity cycling (~5.4 mmol/L increase). Muscle activation was significantly higher with high intensity cycling compared to low intensity cycling with BFR, regardless of pressure (~25% vs. ~12% MVC). Mean power frequency was not different between conditions but did increase from the first 5 minutes of exercise to the last 5 minutes (93% vs. 101%, p < 0.001). Ratings of perceived exertion (RPE) were higher with high intensity cycling but discomfort was similar between conditions. We wish to suggest that high intensity cycling produces greater muscular stress than that observed with work matched low intensity cycling in combination with BFR.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"428-41"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.10","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.5
Roya Naderi, G. Mohaddes, M. Mohammadi, A. Alihemmati, R. Badalzadeh, R. Ghaznavi, R. Ghyasi, S. Mohammadi
Since some complications of diabetes mellitus may be caused or exacerbated by an oxidative stress, the protective effects of garlic (Allium sativum) were investigated in the blood and heart of streptozotocin-induced diabetic rats. Twenty-eight male Wistar rats were randomly divided into four groups: control, garlic, diabetic, and diabetic+garlic. Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (50 mg/kg) in male rats. Rats were fed with raw fresh garlic homogenate (250 mg/kg) six days a week by gavage for a period of 6 weeks. At the end of the 6th week blood samples and heart tissues were collected and used for determination of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and histological evaluation. Induction of diabetes increased MDA levels in blood and homogenates of heart. In diabetic rats treated with garlic, MDA levels decreased in blood and heart homogenates. Treatment of diabetic rats with garlic increased SOD, GPX and CAT in blood and heart homogenates. Histopathological finding of the myocardial tissue confirmed a protective role for garlic in diabetic rats. Thus, the present study reveals that garlic may effectively modulate antioxidants status in the blood and heart of streptozotocin induced-diabetic rats.
{"title":"Preventive effects of garlic (Allium sativum) on oxidative stress and histopathology of cardiac tissue in streptozotocin-induced diabetic rats.","authors":"Roya Naderi, G. Mohaddes, M. Mohammadi, A. Alihemmati, R. Badalzadeh, R. Ghaznavi, R. Ghyasi, S. Mohammadi","doi":"10.1556/036.102.2015.4.5","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.5","url":null,"abstract":"Since some complications of diabetes mellitus may be caused or exacerbated by an oxidative stress, the protective effects of garlic (Allium sativum) were investigated in the blood and heart of streptozotocin-induced diabetic rats. Twenty-eight male Wistar rats were randomly divided into four groups: control, garlic, diabetic, and diabetic+garlic. Diabetes was induced by intraperitoneal (i.p.) injection of streptozotocin (50 mg/kg) in male rats. Rats were fed with raw fresh garlic homogenate (250 mg/kg) six days a week by gavage for a period of 6 weeks. At the end of the 6th week blood samples and heart tissues were collected and used for determination of glutathione peroxidase (GPx), superoxide dismutase (SOD), catalase (CAT), malondialdehyde (MDA) and histological evaluation. Induction of diabetes increased MDA levels in blood and homogenates of heart. In diabetic rats treated with garlic, MDA levels decreased in blood and heart homogenates. Treatment of diabetic rats with garlic increased SOD, GPX and CAT in blood and heart homogenates. Histopathological finding of the myocardial tissue confirmed a protective role for garlic in diabetic rats. Thus, the present study reveals that garlic may effectively modulate antioxidants status in the blood and heart of streptozotocin induced-diabetic rats.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"58 1","pages":"380-90"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.5","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627936","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2015-12-21DOI: 10.1556/036.102.2015.4.11
J. Rubio-Arias, Paula Esteban, F. Martínez, D. Ramos-Campo, Susana Mendizábal, D. Berdejo-del-Fresno, J. Jiménez-Díaz
The applied use of new technologies to enhance performance and improve health has been increasing. Initially, whole body vibration training (WBVT) was used as system to improve elite athlete performance. However, this is also used to improve body composition, especially there is a great attention on the effectiveness of WBVT to reduce fat and body weight, with a potential increase in muscle tissue. The aim of this study was to investigate the effects of a 6-week vibration-training program on total and segmental body composition in a group of physically healthy participants. The final study sample included 64 healthy young adults. Subjects were randomly allocated into the control group (CG: n = 26; 16 males and 10 females) and the experimental group (EGWBVT: n = 38; 19 males and 19 females). The program lasted six weeks with a frequency of three sessions per week and each session varied in intensity. There were not found statistically significant differences in any of the body composition variables analysed. This study suggests that a six-week vibration-training program with an increasing intensity (7.2 g-32.6 g) in healthy young adults that are not overweight did not alter total and segmental body composition.
新技术在提高绩效和改善健康方面的应用越来越多。最初，全身振动训练(WBVT)被用作提高精英运动员成绩的系统。然而，这也被用于改善身体成分，特别是有很大的关注WBVT的有效性，以减少脂肪和体重，潜在的肌肉组织的增加。本研究的目的是调查为期六周的振动训练计划对一组身体健康的参与者的整体和部分身体组成的影响。最终的研究样本包括64名健康的年轻人。受试者随机分为对照组(CG: n = 26;实验组(EGWBVT: n = 38;男性19名，女性19名)。该项目持续了六周，频率为每周三次，每次的强度各不相同。在分析的身体成分变量中，没有发现统计学上的显著差异。这项研究表明，在没有超重的健康年轻人中进行为期六周的振动训练计划，增加强度(7.2 g-32.6 g)，不会改变整体和部分身体组成。
{"title":"Effect of 6 weeks of whole body vibration training on total and segmental body composition in healthy young adults.","authors":"J. Rubio-Arias, Paula Esteban, F. Martínez, D. Ramos-Campo, Susana Mendizábal, D. Berdejo-del-Fresno, J. Jiménez-Díaz","doi":"10.1556/036.102.2015.4.11","DOIUrl":"https://doi.org/10.1556/036.102.2015.4.11","url":null,"abstract":"The applied use of new technologies to enhance performance and improve health has been increasing. Initially, whole body vibration training (WBVT) was used as system to improve elite athlete performance. However, this is also used to improve body composition, especially there is a great attention on the effectiveness of WBVT to reduce fat and body weight, with a potential increase in muscle tissue. The aim of this study was to investigate the effects of a 6-week vibration-training program on total and segmental body composition in a group of physically healthy participants. The final study sample included 64 healthy young adults. Subjects were randomly allocated into the control group (CG: n = 26; 16 males and 10 females) and the experimental group (EGWBVT: n = 38; 19 males and 19 females). The program lasted six weeks with a frequency of three sessions per week and each session varied in intensity. There were not found statistically significant differences in any of the body composition variables analysed. This study suggests that a six-week vibration-training program with an increasing intensity (7.2 g-32.6 g) in healthy young adults that are not overweight did not alter total and segmental body composition.","PeriodicalId":7167,"journal":{"name":"Acta physiologica Hungarica","volume":"102 4 1","pages":"442-50"},"PeriodicalIF":0.0,"publicationDate":"2015-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1556/036.102.2015.4.11","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"67627950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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