The expression profiles of fibroblast growth factor 9 and its receptors in developing mice testes.

IF 1.6 4区生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Organogenesis Pub Date : 2016-04-02 Epub Date: 2016-04-14 DOI:10.1080/15476278.2016.1171448

Meng-Shao Lai, Chia-Yih Wang, Shang-Hsun Yang, Chia-Ching Wu, H Sunny Sun, Shaw-Jenq Tsai, Jih-Ing Chuang, Yung-Chia Chen, Bu-Miin Huang

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引用次数: 10

Abstract

An expressional lack of fibroblast growth factor 9 (FGF9) would cause male-to-female sex reversal in the mouse, implying the essential role of FGF9 in testicular organogenesis and maturation. However, the temporal expression of FGF9 and its receptors during testicular development remains elusive. In this study, immunohistochemistry was used to identify the localization of FGF9 and its receptors at different embryonic and postnatal stages in mice testes. Results showed that FGF9 continuously expressed in the testis during development. FGF9 had highest expression in the interstitial region at 17-18 d post coitum (dpc) and in the spermatocytes, spermatids and Leydig cell on postnatal days (pnd) 35-65. Regarding receptor expression, FGFR1 and FGFR4 were evenly expressed in the whole testis during the embryonic and postnatal stages. However, FGFR2 and FGFR3 were widely expressed during the embryonic testis development with higher FGFR2 expression in seminiferous tubules at 16-18 dpc and higher FGFR3 expression in interstitial region at 17-18 dpc. In postnatal stage, FGFR2 extensively expressed with higher expression at spermatids and Leydig cells on 35-65 pnd and FGFR3 widely expressed in the whole testis. Taken together, these results strongly suggest that FGF9 is correlated with the temporal expression profiles of FGFR2 and FGFR3 and possibly associated with testis development.

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成纤维细胞生长因子9及其受体在发育小鼠睾丸中的表达谱。

在小鼠中，成纤维细胞生长因子9 (FGF9)的表达缺乏会导致雄性向雌性的性别逆转，这意味着FGF9在睾丸器官发生和成熟中起着重要作用。然而，FGF9及其受体在睾丸发育过程中的时间表达尚不清楚。本研究采用免疫组织化学方法鉴定了FGF9及其受体在小鼠睾丸不同胚胎和出生后阶段的定位。结果表明，FGF9在睾丸发育过程中持续表达。FGF9在性交后17 ~ 18 d间质区表达量最高，在产后35 ~ 65 d精母细胞、精母细胞和间质细胞表达量最高。在受体表达方面，FGFR1和FGFR4在胚胎期和出生后的整个睾丸中均匀表达。然而，FGFR2和FGFR3在胚胎睾丸发育过程中广泛表达，在16-18 dpc时，FGFR2在精管中表达较高，在17-18 dpc时，FGFR3在间质区表达较高。在产后，FGFR2广泛表达，在35-65 pnd的精细胞和间质细胞中表达较高，FGFR3在整个睾丸中广泛表达。综上所述，这些结果强烈表明FGF9与FGFR2和FGFR3的时间表达谱相关，并可能与睾丸发育有关。

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来源期刊

Organogenesis BIOCHEMISTRY & MOLECULAR BIOLOGY-DEVELOPMENTAL BIOLOGY

CiteScore

4.10

自引率

4.30%

发文量

审稿时长

>12 weeks

期刊介绍： Organogenesis is a peer-reviewed journal, available in print and online, that publishes significant advances on all aspects of organ development. The journal covers organogenesis in all multi-cellular organisms and also includes research into tissue engineering, artificial organs and organ substitutes. The overriding criteria for publication in Organogenesis are originality, scientific merit and general interest. The audience of the journal consists primarily of researchers and advanced students of anatomy, developmental biology and tissue engineering. The emphasis of the journal is on experimental papers (full-length and brief communications), but it will also publish reviews, hypotheses and commentaries. The Editors encourage the submission of addenda, which are essentially auto-commentaries on significant research recently published elsewhere with additional insights, new interpretations or speculations on a relevant topic. If you have interesting data or an original hypothesis about organ development or artificial organs, please send a pre-submission inquiry to the Editor-in-Chief. You will normally receive a reply within days. All manuscripts will be subjected to peer review, and accepted manuscripts will be posted to the electronic site of the journal immediately and will appear in print at the earliest opportunity thereafter.