Prevalence of Antibiotics Resistant Listeria monocytogenes Strains in Nigerian  Ready-to-eat Foods.

Food safety (Tokyo, Japan) Pub Date : 2018-09-28 eCollection Date: 2018-09-01 DOI:10.14252/foodsafetyfscj.2018002

Daniel O Ebakota, Onilude A Abiodun, Obayagbona O Nosa

{"title":"Prevalence of Antibiotics Resistant Listeria monocytogenes Strains in Nigerian \u2028Ready-to-eat Foods.","authors":"Daniel O Ebakota, Onilude A Abiodun, Obayagbona O Nosa","doi":"10.14252/foodsafetyfscj.2018002","DOIUrl":null,"url":null,"abstract":"The Listerial flora and Listeria monocytogenes bio-load associated with 411 ready-to-eat (RTE) foods sold at several locations in southern Nigeria was evaluated using phenotypic procedures which included serial dilution and pour plate techniques. Selected L. monocytogenes strains phenotypically identified from the RTE foods were further identified using virulence gene markers and 16srRNA amplification procedures. All the 90 L. monocytogenes strains cultured from the RTE foods were subjected to antibiogram analysis using disc diffusion. Some of the antibiotics employed included; Ceftazidine, cefuroxime, gentamicin, ofloxacin, augumentin, tetracycline and erythromycin. L. monocytogenes L. ivanovii, L. grayi, L.welshimeri, L. seeligeri and L. innocua were detected in the RTE foods. Haemolysin (hlyA) gene, Internalin gene (inlA) and invasive gene (iap) were detected in all L. monocytogenes isolates. L. monocytogenes LMEW70 with accession number KY053295 was 93% similar to L. monocytogenes L1846. All the L. monocytogenes isolates were resistant to amoxicillin, cloxacillin, augumentin and ceftazidime.","PeriodicalId":73044,"journal":{"name":"Food safety (Tokyo, Japan)","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2018-09-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.14252/foodsafetyfscj.2018002","citationCount":"8","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food safety (Tokyo, Japan)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14252/foodsafetyfscj.2018002","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2018/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}

引用次数: 8

Abstract

The Listerial flora and Listeria monocytogenes bio-load associated with 411 ready-to-eat (RTE) foods sold at several locations in southern Nigeria was evaluated using phenotypic procedures which included serial dilution and pour plate techniques. Selected L. monocytogenes strains phenotypically identified from the RTE foods were further identified using virulence gene markers and 16srRNA amplification procedures. All the 90 L. monocytogenes strains cultured from the RTE foods were subjected to antibiogram analysis using disc diffusion. Some of the antibiotics employed included; Ceftazidine, cefuroxime, gentamicin, ofloxacin, augumentin, tetracycline and erythromycin. L. monocytogenes L. ivanovii, L. grayi, L.welshimeri, L. seeligeri and L. innocua were detected in the RTE foods. Haemolysin (hlyA) gene, Internalin gene (inlA) and invasive gene (iap) were detected in all L. monocytogenes isolates. L. monocytogenes LMEW70 with accession number KY053295 was 93% similar to L. monocytogenes L1846. All the L. monocytogenes isolates were resistant to amoxicillin, cloxacillin, augumentin and ceftazidime.

Abstract Image

查看原文

微信好友朋友圈 QQ好友复制链接

本刊更多论文

尼日利亚耐抗生素单核细胞增生李斯特菌流行情况 即食食品。

使用表型程序(包括连续稀释和倒盘技术)对尼日利亚南部几个地点销售的411种即食食品相关的李斯特菌群和单核增生李斯特菌生物负荷进行了评估。通过毒力基因标记和16srRNA扩增程序，进一步鉴定从RTE食品中鉴定出的具有表型特征的单核增生乳杆菌菌株。采用圆盘扩散法对从RTE食品中培养的90株单核增生乳杆菌进行抗生素谱分析。使用的一些抗生素包括;头孢他嗪，头孢呋辛，庆大霉素，氧氟沙星，奥古汀，四环素和红霉素。在RTE食品中检出单增李斯特菌伊万诺氏李斯特菌、格雷李斯特李斯特菌、韦氏李斯特李斯特李斯特菌和无尾李斯特李斯特李斯特李斯特李斯特菌。所有单核增生乳杆菌分离株均检测到溶血素(hlyA)基因、内毒素(inlA)基因和侵袭基因(iap)。加入号为KY053295的L. monocytogenes LMEW70与L. monocytogenes L1846相似度为93%。所有单核增生乳杆菌分离株均对阿莫西林、氯西林、奥古汀和头孢他啶耐药。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文去求助

来源期刊