Quantitative assessment of CD15 positive tissue eosinophils in Oral Squamous Cell Carcinoma: effects on mast cells and tumor angiogenesis.

Q3 Medicine Minerva stomatologica Pub Date : 2020-06-01 DOI:10.23736/S0026-4970.19.04285-7

Aashka Sethi, Devi C Shetty, Ajit S Rathore, Ankita Tandon, Saurabh Juneja, Nikita Gulati

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引用次数: 1

Abstract

Background: The present study determines to correlate eosinophil, mast cell and microvessel densities with the histopathological grades and clinical staging of Oral Squamous Cell Carcinoma (OSCC) cases, as the potential role of inflammatory mediators within tumor stroma remains debatable.

Methods: The study sample comprised 60 cases consisting of 40 cases of Well to moderately differentiated OSCC (group 1) and 20 cases of poorly differentiated OSCC (group 2). Immunohistochemistry with anti-CD15 antibody and antifactor VIII antibody; and toluidine blue special stain were employed for the detection of eosinophils, microvessels, and mast cells, respectively.

Results: The mean numbers of eosinophils, mast cells, and microvessels per high power field in group 1 and group 2 were 15.37±11.86 and 12.62±14.30, 6.00±4.84 and 4.51±4.51, 13.96±6.25 and 6.62±2.05, respectively. Eosinophil density had a positive correlation with both mast cell and microvessel density. Also, the correlation of primary tumor size (T status) with microvessel density was found to be statistically significant (P≤0.05).

Conclusions: The cohesive interpretation of the aforementioned mediators in OSCC suggested that while these variables correlate well with the differentiation of tumor, the quantification did not correlate with the clinical staging of the disease.

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口腔鳞状细胞癌中CD15阳性组织嗜酸性粒细胞的定量评估:对肥大细胞和肿瘤血管生成的影响。

背景:本研究确定了嗜酸性粒细胞、肥大细胞和微血管密度与口腔鳞状细胞癌(OSCC)病例的组织病理学分级和临床分期的相关性，因为炎症介质在肿瘤基质中的潜在作用仍有争议。方法:选取60例OSCC，其中高分化至中分化OSCC 40例(1组)，低分化OSCC 20例(2组)。免疫组化检测抗cd15抗体和抗因子VIII抗体;用甲苯胺蓝专用染色分别检测嗜酸性粒细胞、微血管和肥大细胞。结果:1组和2组每高倍视场平均嗜酸性粒细胞、肥大细胞和微血管数目分别为15.37±11.86和12.62±14.30,6.00±4.84和4.51±4.51,13.96±6.25和6.62±2.05。嗜酸性粒细胞密度与肥大细胞和微血管密度均呈正相关。原发肿瘤大小(T状态)与微血管密度的相关性也有统计学意义(P≤0.05)。结论:上述介质在OSCC中的内聚性解释表明，虽然这些变量与肿瘤的分化有很好的相关性，但量化与疾病的临床分期无关。

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来源期刊

Minerva stomatologica DENTISTRY, ORAL SURGERY & MEDICINE-

CiteScore

1.50

自引率

0.00%

发文量

期刊介绍： The journal Minerva Stomatologica publishes scientific papers on dentistry and maxillo-facial surgery. Manuscripts may be submitted in the form of editorials, original articles, review articles, case reports, therapeutical notes, special articles and letters to the Editor. Manuscripts are expected to comply with the instructions to authors which conform to the Uniform Requirements for Manuscripts Submitted to Biomedical Editors by the International Committee of Medical Journal Editors (www.icmje.org). Articles not conforming to international standards will not be considered for acceptance.