One Pot Cooking of Rice Grains for Preparation of Rice-Gel Samples Using a Small-Scale Viscosity Analyzer: Small-scale Rice-gel Preparation from Grains.

IF 1.2 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY Journal of applied glycoscience Pub Date : 2019-11-20 eCollection Date: 2019-01-01 DOI:10.5458/jag.jag.JAG-2019_0009
Junko Matsuki, Tomoko Sasaki, Koichi Yoza, Junichi Sugiyama, Hideo Maeda, Ken Tokuyasu
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引用次数: 4

Abstract

Rice-gel prepared by the following three steps: rice grain cooking, shearing of the cooked rice, and cooling for gel formation, is expected as a novel food ingredient for modification of various food products such as bread and noodles. To meet the demand for high-throughput systems for research and developments on the new rice gels, herein we established a mini-cooking system for preparation of rice gel samples from grains using a small-scale viscosity analyzer (Rapid Visco Analyzer; RVA). Polished rice grains (4 g) were cooked with 22 mL of water in a canister, and the paddle equipped in the canister was rotated at 2,000 rpm for 30 min (80 °C was used as a representative) to shear the cooked rice. The sheared paste was cooled to 10 °C at 160 rpm, and the initial gelation property was evaluated by viscosity analysis within the RVA. Alternatively, the sheared paste was transferred to an acrylic mold and kept at 4 °C for 0, 1, 3, and 5 days for determination of the hardness with a compression test. Compressive forces required to penetrate 20 % thickness for three tested rice cultivars were measured, and the trend of the value shifts during preservation is similar to the corresponding trend obtained in 300-g grain scale laboratory tests, whereas the individual values were halved in the former. This small cooking method could offer a useful assay system for a rapid evaluation in the breeding programs and in the high-throughput screening of additives for the modification of properties.

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一锅蒸煮米粒制备凝胶样品的小型粘度分析仪:用米粒制备小规模凝胶。
米凝胶是通过以下三个步骤制备的:米粒煮熟,煮熟的米剪切,冷却形成凝胶,有望成为一种新的食品原料,用于各种食品的改性,如面包和面条。为了满足新型米凝胶研究和开发对高通量系统的需求,我们建立了一个小型烹饪系统,使用小型粘度分析仪(Rapid Visco analyzer;RVA)。精米粒(4 g)用22 mL的水在罐中煮熟,罐中配备的桨以2000 rpm转速旋转30 min(以80℃为代表)剪切熟米。剪切后的膏体在160 rpm的转速下冷却至10°C,通过RVA内的粘度分析来评估初始凝胶特性。或者,将剪切后的膏体转移到丙烯酸模具中,在4°C下保存0、1、3和5天,通过压缩测试来确定硬度。测定了3个水稻品种穿透20%厚度所需的压缩力,结果表明,在保存过程中,压缩力值的变化趋势与300 g粒级室内试验的变化趋势相似,而前者的个体值则减半。这种小蒸煮方法可以为育种方案的快速评价和性状改性添加剂的高通量筛选提供一种有用的检测系统。
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来源期刊
Journal of applied glycoscience
Journal of applied glycoscience BIOCHEMISTRY & MOLECULAR BIOLOGY-
自引率
9.10%
发文量
13
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