Antimicrobial Drug-resistance Profile of Vibrio Parahaemolyticus isolated from Japanese Horse Mackerel (Trachurus Japonicus).

Food safety (Tokyo, Japan) Pub Date : 2021-09-24 eCollection Date: 2021-09-01 DOI:10.14252/foodsafetyfscj.D-21-00001
Tasturo Nishino, Hideki Suzuki, Shiro Mizumoto, Hirotaka Morinushi, Hiromi Nagaoka, Keiichi Goto, Shigeki Yamamoto
{"title":"Antimicrobial Drug-resistance Profile of <i>Vibrio Parahaemolyticus</i> isolated from Japanese Horse Mackerel (<i>Trachurus Japonicus</i>).","authors":"Tasturo Nishino,&nbsp;Hideki Suzuki,&nbsp;Shiro Mizumoto,&nbsp;Hirotaka Morinushi,&nbsp;Hiromi Nagaoka,&nbsp;Keiichi Goto,&nbsp;Shigeki Yamamoto","doi":"10.14252/foodsafetyfscj.D-21-00001","DOIUrl":null,"url":null,"abstract":"<p><p>This study aimed at investigating antimicrobial resistance (AMR) profile of <i>Vibrio parahaemolyticus (V. parahaemolyticus).</i> The bacteria were isolated from wild-caught and farmed Japanese horse mackerel (<i>Trachurus japonicus</i>), and examined for the antimicrobial drug resistance. Furthermore, the serotype, and the genes of thermostable direct hemolysin (<i>tdh</i>) and cholera toxin transcriptional activator (<i>toxR</i>) of the isolates were investigated by using a serotype testing kit and PCR method. Eighty-eight and 126 <i>V. parahaemolyticus</i> strains were isolated from wild-caught and farmed Japanese horse mackerel, respectively. Ten and 18 distinct serotypes were detected from wild-caught and farmed Japanese horse mackerel. All strains were negative for <i>tdh</i> genes but positive for <i>toxR</i> genes. Resistances to ampicillin (ABP) and to both ABP and fosfomycin (FOM) were observed in 54 and 23 strains from the wild-caught fish, while those resistant strains from farm fish were 112 and 7 strains. Multidrug-resistance to three or four drugs including ABP was observed in one or two strains from the wild-caught fish. These results strongly suggest that the environmental exposure of antimicrobial drugs results in the spread of resistant genes in Japanese horse mackerel. This study highlights the need for monitoring the spread of resistance genes to the human intestinal flora as well as to other bacteria in the environment.</p>","PeriodicalId":73044,"journal":{"name":"Food safety (Tokyo, Japan)","volume":"9 3","pages":"75-80"},"PeriodicalIF":0.0000,"publicationDate":"2021-09-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8472095/pdf/","citationCount":"2","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food safety (Tokyo, Japan)","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.14252/foodsafetyfscj.D-21-00001","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2021/9/1 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 2

Abstract

This study aimed at investigating antimicrobial resistance (AMR) profile of Vibrio parahaemolyticus (V. parahaemolyticus). The bacteria were isolated from wild-caught and farmed Japanese horse mackerel (Trachurus japonicus), and examined for the antimicrobial drug resistance. Furthermore, the serotype, and the genes of thermostable direct hemolysin (tdh) and cholera toxin transcriptional activator (toxR) of the isolates were investigated by using a serotype testing kit and PCR method. Eighty-eight and 126 V. parahaemolyticus strains were isolated from wild-caught and farmed Japanese horse mackerel, respectively. Ten and 18 distinct serotypes were detected from wild-caught and farmed Japanese horse mackerel. All strains were negative for tdh genes but positive for toxR genes. Resistances to ampicillin (ABP) and to both ABP and fosfomycin (FOM) were observed in 54 and 23 strains from the wild-caught fish, while those resistant strains from farm fish were 112 and 7 strains. Multidrug-resistance to three or four drugs including ABP was observed in one or two strains from the wild-caught fish. These results strongly suggest that the environmental exposure of antimicrobial drugs results in the spread of resistant genes in Japanese horse mackerel. This study highlights the need for monitoring the spread of resistance genes to the human intestinal flora as well as to other bacteria in the environment.

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
日本马鲛鱼副溶血性弧菌的耐药性分析。
本研究旨在研究副溶血性弧菌(V. parahaemolyticus)的抗微生物药物耐药性(AMR)。从野生和养殖的日本马鲛鱼(Trachurus japonicus)中分离出细菌,并进行了抗菌药物耐药性检测。此外,采用血清型检测试剂盒和PCR方法对分离株进行了血清型检测,并对热稳定型直接溶血素(tdh)和霍乱毒素转录激活因子(toxR)基因进行了检测。从野生马鲛鱼和养殖马鲛鱼中分别分离到88株和126株副溶血性弧菌。从野生和养殖的日本马鲛鱼中分别检测到10种和18种不同的血清型。所有菌株tdh基因均阴性,而toxR基因均阳性。对氨苄西林(ABP)和磷霉素(FOM)均耐药的野生鱼有54株和23株,对养殖鱼有112株和7株。在野生捕获的鱼类中发现1 ~ 2株对包括ABP在内的3 ~ 4种药物具有多重耐药。这些结果有力地表明,抗菌药的环境暴露导致了日本马鲛鱼耐药基因的传播。这项研究强调了监测耐药基因在人类肠道菌群以及环境中其他细菌中的传播的必要性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
自引率
0.00%
发文量
0
期刊最新文献
Cadmium (Third Edition) (Chemicals and Contaminants). Geographical Variation of Antimicrobial Resistance of Salmonella in Japanese Chicken. 3-Nitrooxypropanol (Feed Additives). Quinofumelin (Pesticides). Basic Principles for Setting MRLs for Pesticides in Food Commodities in Japan.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1