Role of lncRNA LIPE-AS1 in adipogenesis.

IF 3.5 4区生物学 Q2 ENDOCRINOLOGY & METABOLISM Adipocyte Pub Date : 2022-12-01 DOI:10.1080/21623945.2021.2013415

Alyssa Thunen, Deirdre La Placa, Zhifang Zhang, John E Shively

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引用次数: 10

Abstract

Recent studies have identified long non-coding RNAs (lncRNAs) as potential regulators of adipogenesis. In this study, we have characterized a lncRNA, LIPE-AS1, that spans genes CEACAM1 to LIPE in man with conservation of genomic organization and tissue expression between mouse and man. Tissue-specific expression of isoforms of the murine lncRNA were found in liver and adipose tissue, one of which, designated mLas-V3, overlapped the Lipe gene encoding hormone-sensitive lipase in both mouse and man suggesting that it may have a functional role in adipose tissue. Knock down of expression of mLas-V3 using anti-sense oligos (ASOs) led to a significant decrease in the differentiation of the OP9 pre-adipocyte cell line through the down regulation of the major adipogenic transcription factors Pparg and Cebpa. Knock down of mLas-V3 induced apoptosis during the differentiation of OP9 cells as shown by expression of active caspase-3, a change in the localization of LIP/LAP isoforms of C/EBPβ, and expression of the cellular stress induced factors CHOP, p53, PUMA, and NOXA. We conclude that mLas-V3 may play a role in protecting against stress associated with adipogenesis, and its absence leads to apoptosis.

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lncRNA LIPE-AS1在脂肪形成中的作用。

最近的研究发现长链非编码rna (lncRNAs)是脂肪形成的潜在调节因子。在这项研究中，我们鉴定了一个lncRNA LIPE- as1，它跨越了人类基因CEACAM1到LIPE，在小鼠和人类之间具有基因组组织和组织表达的保守性。在肝脏和脂肪组织中发现了小鼠lncRNA的组织特异性表达，其中命名为mLas-V3的lncRNA与小鼠和人类中编码激素敏感脂肪酶的Lipe基因重叠，这表明它可能在脂肪组织中具有功能作用。利用反义寡核苷酸(ASOs)敲低mLas-V3的表达，通过下调主要的脂肪生成转录因子Pparg和Cebpa，导致OP9前脂肪细胞系的分化显著减少。通过活性caspase-3的表达、C/EBPβ的LIP/LAP亚型的定位变化以及细胞应激诱导因子CHOP、p53、PUMA和NOXA的表达，表明mLas-V3的敲低诱导OP9细胞在分化过程中凋亡。我们得出结论，mLas-V3可能在与脂肪形成相关的应激中起保护作用，其缺失会导致细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Adipocyte Medicine-Histology

CiteScore

6.50

自引率

3.00%

发文量

审稿时长

32 weeks

期刊介绍： Adipocyte recognizes that the adipose tissue is the largest endocrine organ in the body, and explores the link between dysfunctional adipose tissue and the growing number of chronic diseases including diabetes, hypertension, cardiovascular disease and cancer. Historically, the primary function of the adipose tissue was limited to energy storage and thermoregulation. However, a plethora of research over the past 3 decades has recognized the dynamic role of the adipose tissue and its contribution to a variety of physiological processes including reproduction, angiogenesis, apoptosis, inflammation, blood pressure, coagulation, fibrinolysis, immunity and general metabolic homeostasis. The field of Adipose Tissue research has grown tremendously, and Adipocyte is the first international peer-reviewed journal of its kind providing a multi-disciplinary forum for research focusing exclusively on all aspects of adipose tissue physiology and pathophysiology. Adipocyte accepts high-profile submissions in basic, translational and clinical research.