Glyphosate disrupts sperm quality and induced DNA damage of rainbow trout (Oncorhynchus mykiss) sperm.

IF 1.2 4区 环境科学与生态学 Q4 ENVIRONMENTAL SCIENCES Journal of Environmental Science and Health Part C-Toxicology and Carcinogenesis Pub Date : 2021-01-01 Epub Date: 2021-09-01 DOI:10.1080/26896583.2021.1969180
Ayşe Akça, Mehmet Kocabaş, Filiz Kutluyer
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引用次数: 2

Abstract

As a widespread pollutant, glyphosate (GLY) adversely affects the aquatic environment and can impair the reproductive ability and functions of fish. The purpose of the current study was to assess in vitro effect of GLY on rainbow trout (Oncorhynchus mykiss) sperm cells. The sperm cells were exposed to different GLY concentrations (2.5, 5, 10 mg/L). Sperm motility parameters were analyzed with computer assisted sperm analysis. DNA fragmentation (%) was measured by the comet assay using fluorescence microscopy. With increased GLY concentration, sperm motility and duration decreased after exposure. DNA fragmentation (% DNA in tail) in sperm cells was higher in treatments containing GLY than control (p < 0.05). Consequently, sperm cells are sensitive to low doses of GLY, and this can negatively affect natural populations.

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草甘膦破坏虹鳟精子质量,引起虹鳟精子DNA损伤。
草甘膦(glyphosate, GLY)是一种广泛存在的污染物,对水生环境产生不利影响,损害鱼类的生殖能力和功能。本研究的目的是评估GLY对虹鳟精子细胞的体外影响。精子细胞暴露于不同浓度的GLY(2.5、5、10 mg/L)中。用计算机辅助精子分析分析精子活力参数。采用彗星荧光显微镜测定DNA片段率(%)。随着GLY浓度的增加,暴露后精子活力和持续时间下降。含GLY处理的精子细胞DNA片段率(尾部DNA百分比)高于对照组(p
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CiteScore
4.60
自引率
0.00%
发文量
10
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