Evaluation of sialic acid, malondialdehyde and glutathione levels in infertile male

IF 0.8 4区医学 Q4 ANDROLOGY Revista Internacional De Andrologia Pub Date : 2022-10-01 DOI:10.1016/j.androl.2021.05.002

Esma Menevse , Duygu Dursunoglu , Nihal Cetin , Emine Nedime Korucu , Fatma Zehra Erbayram

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引用次数: 1

Abstract

Introduction and objectives

Around 15% of productive couples in the world are infertile. Recent years, biochemical mechanisms leads to male infertility are started to research. Redox regulation and oxidative stress (OS) show importance in the pathogenesis of infertility in male. Malondialdehyde (MDA) and Glutathione (GSH) are biochemical indicatives of sperm damage by reactive oxygen species (ROS). In addition, sperm are coated with a thick glycocalyx rich in sialic acids. It is aimed to determine and evaluate the differences between normozoospermic and oligozoospermic individuals according to sialic acid, MDA and GSH concentrations and correlations between spermyogram and these parameters.

Material and methods

This study was carried out on seminal plasma of individuals who admitted to Selcuk University Faculty of Medicine IVF Unit Andrology Laboratory. The groups were divided into two as normozoospermics (n = 30, sperm concentration ≥ 15 million/mL), and oligozoospermics (n = 30, sperm concentration < 15 million/mL). Spermyogram were evaluated regarding WHO (2010) Kruger criteria. GSH, MDA and sialic acid concentrations were analyzed in seminal plasma. Diagnostic performance of sialic acid has been determined with ROC curve analysis.

Results

Sialic acid levels were significantly lower in Normozoospermic than Oligozoospermic individuals (p < 0.0001), MDA and GSH levels were not differ significantly in both groups (p > 0.05). Sialic acid correlated significantly with most of the spermyogram findings. When diagnostic performance of sialic acid was evaluated, the cut off value of sialic acid found as 4.175 nmol/mL by ROC curve.

Conclusion

High seminal plasma sialic acid levels may be used as a biomarker and sialic acid is important determinant in oligozoospermia.

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不育男性唾液酸、丙二醛和谷胱甘肽水平的评价

引言和目标世界上有生育能力的夫妇中约有15%是不育的。近年来，人们开始研究导致男性不育的生化机制。氧化还原调控和氧化应激(OS)在男性不育症的发病机制中具有重要意义。丙二醛(MDA)和谷胱甘肽(GSH)是精子受到活性氧(ROS)损伤的生化指标。此外，精子被一层厚厚的富含唾液酸的糖萼所包裹。目的是根据唾液酸、丙二醛和谷胱甘肽的浓度以及精子图与这些参数的相关性来确定和评价正常精子和少精子个体之间的差异。材料和方法本研究是在Selcuk大学医学院体外受精单元男科实验室接受治疗的个体的精液中进行的。正常精子组(n = 30，精子浓度≥1500万/mL)和少精子组(n = 30，精子浓度<1500万/毫升)。根据世卫组织(2010)克鲁格标准对精子图进行评估。测定精浆GSH、MDA和唾液酸浓度。用ROC曲线分析确定唾液酸的诊断性能。结果正常精子组唾液酸水平明显低于少精子组(p <0.0001)，两组间MDA和GSH水平无显著差异(p >0.05)。唾液酸与大多数精肌图结果显著相关。在评价唾液酸的诊断性能时，通过ROC曲线发现唾液酸的截断值为4.175 nmol/mL。结论高精液唾液酸水平可作为少精症的生物标志物，唾液酸是少精症的重要决定因素。

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来源期刊

Revista Internacional De Andrologia ANDROLOGY-

CiteScore

1.60

自引率

12.50%

发文量

审稿时长

>12 weeks

期刊介绍： Revista Internacional de Andrología es la revista oficial de la Asociación Española de Andrología, Medicina Sexual y Reproductiva (ASESA), la Sociedade Portuguesa de Ardrologia, la Sociedad Argentina de Andrología (SAA), la Asociación Iberoamericana de Sociedades de Andrología (ANDRO), y la Federación Española de Sociedades de Sexología. La revista publicada trimestralmente es revisada por pares y es líder en el la especialidad y en español y portugués. Recientemente también publica artículos en inglés. El objetivo de la revista es principalmente la promoción del conocimiento y la educación médica continua, con un enfoque especial en el público español y latinoamericano, a través de la publicación de las contribuciones importantes de la investigación en el campo. Todos los miembros de las sociedades antes mencionadas reciben la revista y otros suscriptores individuales e institucionales de España, Portugal y América Latina.