Neuroprotective Effect of Ethanolic Extract of Scoparia dulcis on Acrylamide-Induced Neurotoxicity in Zebrafish Model

Abstract

All herbal medicines are reported to be safe and have better results in curing disabilities. Scoparia dulcis is known for its anti-inflammatory and antioxidant properties. This study has been executed to explore the neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide using adult zebrafish. The experimental period was 72 h. After fixing the optimum acrylamide concentration and EESD, the healthy adult fish were grouped into control, induction, and treatment. During the experimental period, behavioural changes such as memory and locomotion were observed in control and experimental groups using the T-maze experiment. After 72 h, the neuronal tissues were isolated from the grouped fishes and analysed for various biochemical and enzymatic assays. The mRNA of the HSP-70 gene in control and experimental groups was expressed using RT-PCR. The optimum dosages for acrylamide and EESD were found to be 0.75 mM and 20 µg/mL, respectively. Memory improvement was observed in S. dulcis-treated fish, compared to the acrylamide-treated group using the T-maze assay. The extract reduced the toxicity induced by acrylamide from the various biochemical and histopathological parameters. The result shows the potential neuroprotective effects of ethanolic extract of Scoparia dulcis (EESD) against acrylamide-induced neurotoxicity in adult zebrafish. Therefore, Scoparia dulcis is a potent neuroprotective agent.

Graphical Abstract