Cytotoxic and genotoxic profiles of the pyrethroid insecticide lambda-cyhalothrin and its microformulation Karate® in CHO-K1 cells

IF 2.3 4区医学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Mutation research. Genetic toxicology and environmental mutagenesis Pub Date : 2023-10-01 DOI:10.1016/j.mrgentox.2023.503682

Milagros R.R. Laborde , Marcelo L. Larramendy , Sonia Soloneski

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Abstract

Lambda-cyhalothrin (LCT) and its microformulation Karate® (25 % a.i.) were analysed for its genotoxicity and cytotoxicity on Chinese hamster ovary (CHO-K1) cells. Cytokinesis-block micronucleus cytome (CBMN-cyt) and alkaline single-cell gel electrophoresis (SCGE) bioassays were selected to test genotoxicity. Neutral red uptake (NRU), succinic dehydrogenase activity (MTT) and apoptogenic induction were employed for estimating cytotoxicity. Both compounds were analysed within a concentration range of 0.1–100 µg/mL. Only LCT produced a significant augment in the frequency of micronuclei (MNs) when the cultures were exposed to highest concentrations of 10 and 100 µg LCT/mL. A noticeable decrease in NDI was observed for cultures treated with LCT at 10 and 100 µg/mL. Karate® induced the inhibition of both the proportion of viable cells and succinic dehydrogenase activity and triggered apoptosis 24 h of exposition. Whilst an increased GDI in CHO-K1 cells was observed in the treatments with 1–100 µg Karate®/mL, the GDI was not modified in the treatments employing LCT at equivalent doses. SCGE showed that Karate® was more prone to induce genotoxic effects than LCT. Only 50 µg/mL of Karate® was able to increase apoptosis. Our results demonstrate the genomic instability and cytotoxic effects induced by this pyrethroid insecticide, confirming that LCT exposure can result in a severe drawback for the ecological equilibrium of the environment.

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拟除虫菊酯类杀虫剂λ-氯氟氰菊酯及其微制剂Karate®在CHO-K1细胞中的细胞毒性和遗传毒性特征。

分析了Lambda氯氟氰菊酯（LCT）及其微制剂Karate®（25%a.i.）对中国仓鼠卵巢（CHO-K1）细胞的遗传毒性和细胞毒性。选择细胞分裂阻断微核细胞仪（CBMN-cyt）和碱性单细胞凝胶电泳（SCGE）生物测定法检测遗传毒性。采用中性红摄取（NRU）、琥珀酸脱氢酶活性（MTT）和凋亡诱导来评估细胞毒性。在0.1-100µg/mL的浓度范围内分析了这两种化合物。当培养物暴露于最高浓度为10和100µg LCT/mL时，只有LCT能显著增加微核（MNs）的频率。在用10和100µg/mL的LCT处理的培养物中，观察到NDI显著降低。Karate®诱导活细胞比例和琥珀酸脱氢酶活性的抑制，并在暴露24小时后引发细胞凋亡。虽然在使用1-100µg Karate®/mL的处理中观察到CHO-K1细胞中的GDI增加，但在使用同等剂量的LCT的处理中，GDI没有改变。SCGE显示Karate®比LCT更容易诱发基因毒性效应。只有50µg/mL的Karate®能够增加细胞凋亡。我们的研究结果证明了这种拟除虫菊酯类杀虫剂诱导的基因组不稳定性和细胞毒性作用，证实了LCT暴露会导致环境生态平衡的严重缺陷。

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来源期刊

Mutation research. Genetic toxicology and environmental mutagenesis 生物-毒理学

CiteScore

3.80

自引率

5.30%

发文量

审稿时长

105 days

期刊介绍： Mutation Research - Genetic Toxicology and Environmental Mutagenesis (MRGTEM) publishes papers advancing knowledge in the field of genetic toxicology. Papers are welcomed in the following areas: New developments in genotoxicity testing of chemical agents (e.g. improvements in methodology of assay systems and interpretation of results). Alternatives to and refinement of the use of animals in genotoxicity testing. Nano-genotoxicology, the study of genotoxicity hazards and risks related to novel man-made nanomaterials. Studies of epigenetic changes in relation to genotoxic effects. The use of structure-activity relationships in predicting genotoxic effects. The isolation and chemical characterization of novel environmental mutagens. The measurement of genotoxic effects in human populations, when accompanied by quantitative measurements of environmental or occupational exposures. The application of novel technologies for assessing the hazard and risks associated with genotoxic substances (e.g. OMICS or other high-throughput approaches to genotoxicity testing). MRGTEM is now accepting submissions for a new section of the journal: Current Topics in Genotoxicity Testing, that will be dedicated to the discussion of current issues relating to design, interpretation and strategic use of genotoxicity tests. This section is envisaged to include discussions relating to the development of new international testing guidelines, but also to wider topics in the field. The evaluation of contrasting or opposing viewpoints is welcomed as long as the presentation is in accordance with the journal''s aims, scope, and policies.