BBX7 interacts with BBX8 to accelerate flowering in chrysanthemum.

IF 10.6 Q1 HORTICULTURE Molecular Horticulture Pub Date : 2023-04-01 DOI:10.1186/s43897-023-00055-2

Yiwen Zhai, Yuqing Zhu, Qi Wang, Guohui Wang, Yao Yu, Lijun Wang, Tao Liu, Shenhui Liu, Qian Hu, Sumei Chen, Fadi Chen, Jiafu Jiang

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Abstract

The quantitative control of FLOWERING LOCUS T (FT) activation is important for the floral transition in flowering plants. However, the flowering regulation mechanisms in the day-neutral, summer-flowering chrysanthemum plant remain unclear. In this study, the chrysanthemum BBX7 homolog CmBBX7 was isolated and its flowering function was identified. The expression of CmBBX7 showed a diurnal rhythm and CmBBX7 exhibited higher expression levels than CmBBX8. Overexpression of CmBBX7 in transgenic chrysanthemum accelerated flowering, whereas lines transfected with a chimeric repressor (pSRDX-CmBBX7) exhibited delayed flowering. Yeast single hybridization, luciferase, electrophoretic mobility shift, and chromatin immunoprecipitation assays showed that CmBBX7 directly targets CmFTL1. In addition, we found that CmBBX7 and CmBBX8 interact to positively regulate the expression of CmFTL1 through binding to its promoter. Collectively, these results highlight CmBBX7 as a key cooperator in the BBX8-FT module to control chrysanthemum flowering.

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BBX7与BBX8相互作用，促进菊花开花。

开花位点T（FT）激活的定量控制对于开花植物的花过渡是重要的。然而，夏花菊花植株的开花调控机制尚不清楚。本研究分离了菊花BBX7同源物CmBBX7，并对其开花功能进行了鉴定。CmBBX7的表达显示出昼夜节律，并且CmBBX7表现出比CmBBX8更高的表达水平。CmBBX7在转基因菊花中的过表达加速了开花，而用嵌合阻遏物（pSRDX-CmBBX7）转染的系表现出延迟开花。酵母单杂交、萤光素酶、电泳迁移率偏移和染色质免疫沉淀分析表明，CmBBX7直接靶向CmFTL1。此外，我们发现CmBBX7和CmBBX8相互作用，通过与启动子结合，正向调节CmFTL1的表达。总之，这些结果突出了CmBBX7作为BBX8-FT模块中控制菊花开花的关键合作者。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Molecular Horticulture horticultural research-

CiteScore

8.00

自引率

0.00%

发文量

审稿时长

12 weeks

期刊介绍： Aims Molecular Horticulture aims to publish research and review articles that significantly advance our knowledge in understanding how the horticultural crops or their parts operate mechanistically. Articles should have profound impacts not only in terms of high citation number or the like, but more importantly on the direction of the horticultural research field. Scope Molecular Horticulture publishes original Research Articles, Letters, and Reviews on novel discoveries on the following, but not limited to, aspects of horticultural plants (including medicinal plants): ▪ Developmental and evolutionary biology ▪ Physiology, biochemistry and cell biology ▪ Plant-microbe and plant-environment interactions ▪ Genetics and epigenetics ▪ Molecular breeding and biotechnology ▪ Secondary metabolism and synthetic biology ▪ Multi-omics dealing with data sets of genome, transcriptome, proteome, metabolome, epigenome and/or microbiome. The journal also welcomes research articles using model plants that reveal mechanisms and/or principles readily applicable to horticultural plants, translational research articles involving application of basic knowledge (including those of model plants) to the horticultural crops, novel Methods and Resources of broad interest. In addition, the journal publishes Editorial, News and View, and Commentary and Perspective on current, significant events and topics in global horticultural fields with international interests.