COMPARİSON OF EGG WHİTE AND Ε-POLYCAPROLACTONE FOR THREE-DİMENSİONAL CELL CULTURE

Abstract

It is increasingly becoming important to develop three-dimensional (3-D) cell culture systems due to their numerous advantages over traditional monolayer culture. The aim of this study is to investigate the interaction of adipose derived stem cells (ADSCs) with scaffolds composed of ε-polycaprolactone (ε-PCL) and egg white. In our study; ε-PCL and egg white scaffolds were fabricated from their monomers under the catalysis of tin octoate and by polymerization by heat respectively. Characterization of PCL was carried out with Gel permeability chromatography (GPC), Fourier Transform Infrared Spectrophotometry (FTIR), Proton Nuclear Magnetic Resonance (H-NMR), Differential Scan Calorimetry (DSC) and Scanning Electron Microscopy (SEM). CM-DiI labeled ADSCs were cultured for 12 days on egg white and ε-PCL scaffolds. Cell viability was performed using MTT and nitric oxide level was evaluated for toxicity. Results showed that the number of ADSCs on egg white scaffold increased periodically throughout 12 days compared with the other groups. Although the number of ADSCs on ε-PCL scaffold increased until the 6th day of the culture, the cell number began to decrease after day 6.. These results were associated with the decomposition of PCL scaffolds that occurs through catabolic reactions. This causes the release of lactic acid which makes toxic effect on the cells. Thus, these results showed that egg white scaffold increases and protects cell adhesion and cell viability more than ε-Polycaprolactone scaffold, thus it can be used as a scaffold in tissue engineering studies that involve stem cells.