Bioassay Guided-isolation of Phytochemicals and Their Biological Effects of Solvent Extracts of Dill (Anethum Graveolens) Aerial Portions

IF 1.2 Q4 PHARMACOLOGY & PHARMACY Journal of Herbs, Spices and Medicinal Plants Pub Date : 2021-11-03 DOI:10.1080/10496475.2021.1989734

Nichakan Peerakam, Sanit Thongnest, Prapart Phoowiang, Kwanchayanawish Machana

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引用次数: 1

Abstract

ABSTRACT Crude EtO1 (ethanol maceration) obtained from aerial portions of dill was divided and eluted by solvents, to obtain PEt (petroleum ether), Eth (ethyl acetate) and EtO2 (ethanol) fractions, evaluated for total phenolics and flavonoids and antioxidant (DPPH, ABTS and FRAP assay), anti-protein denaturation, and anti-enzymatic (tyrosinase and collagenase) activities. The PEt extract was separated by column chromatography, while the chemical composition and biological effects were examined by GC-MS. All the extracts contained phenolics and flavonoids and demonstrated antioxidant activities in all assays. PEt extracts showed anti-protein denaturation (IC50 40.92 µg mL−1). A potential of collagenase inhibitor (92.35–98.69%) similar to Epigallocatechin gallate (EGCG) was noted in all extracts. Five compounds (C3, C5A, C5B2b, C9-1 and C9C9 eluted from Pet extract) represented fatty acid and fatty alcohol (nonacosan-10-one, hexadecanoic acid, ethyl ester, octacosanol, 1-hexacosanol, and hexacosanol) as major components and demonstrated inhibition of protein-denaturation (22.85–42.35% except C9-1). The compounds C9C9 and C9-1 demonstrated collagenase inhibitor effect.

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莳萝(Anethum Graveolens)空中部分溶剂提取物的生物测定指导分离及其生物效应

摘要：将从dill地上部分获得的粗EtO1（乙醇浸渍）分离并用溶剂洗脱，以获得PEt（石油醚）、Eth（乙酸乙酯）和EtO2（乙醇）级分，评估总酚类和黄酮类化合物以及抗氧化剂（DPPH、ABTS和FRAP测定）、抗蛋白变性和抗酶活性（酪氨酸酶和胶原酶）。用柱色谱法分离PEt提取物，用气相色谱-质谱法检测其化学成分和生物效应。所有提取物均含有酚类和黄酮类化合物，并在所有测定中显示出抗氧化活性。PEt提取物显示出抗蛋白质变性作用（IC50 40.92µg mL−1）。在所有提取物中都发现了类似于表没食子儿茶素没食子酸盐（EGCG）的胶原酶抑制剂的潜力（92.35-98.69%）。五种化合物（从Pet提取物中洗脱的C3、C5A、C5B2b、C9-1和C9C9）代表脂肪酸和脂肪醇（壬烷-10酮、十六烷酸、乙酯、二十八烷醇、1-二十六烷醇和二十六烷醇）为主要成分，并表现出对蛋白质变性的抑制作用（除C9-1外，22.85-42.35%）。化合物C9C9和C9-1表现出胶原酶抑制剂的作用。

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来源期刊

Journal of Herbs, Spices and Medicinal Plants Medicine-Complementary and Alternative Medicine

CiteScore

3.00

自引率

0.00%

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期刊介绍： The Journal of Herbs, Spices & Medicinal Plants is an essential reference filled with recent research and other valuable information associated with herbs, spices, and medicinal plants. The Journal serves as a focus point through which investigators and others may publish material of importance to the production, marketing, and utilization of these plants and associated extracts. The journal covers the following topics: growth, development, horticulture, ecology, physiology, genetics, chemistry, and economics. Original articles, review articles, and book reviews provide information of interest to an international audience of researchers, teachers, technicians, and managers involved with production and/or marketing of herbs, spices, and medicinal plants. Managers of food companies, food processing facilities, medical research laboratories, government agencies, and others interested in new chemicals, food additives, international trade, patents, and other items can easily review new findings. The Journal of Herbs, Spices & Medicinal Plants is a forum in which recent research and other information associated with herbs, spices, and medicinal plants is shared. The Journal represents a centralized database accessible by investigators within the international community that work with or have an interest in herbs, spices, and medicinal plants.