Ontogenetic and expression of different genes involved in the Toll pathway of black tiger shrimp (Penaeus monodon) following immersion challenge with Vibrio harveyi and white spot syndrome virus (WSSV)

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2018-06-01 DOI:10.1016/j.aggene.2018.05.002
K. Sreedharan, A. Deepika, Anutosh Paria, M.K. Bedekar, M. Makesh, K.V. Rajendran
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引用次数: 6

Abstract

In the present study, ontogenetic expression of different innate immune genes in the Toll pathway of tiger shrimp, Penaeus monodon, such as TLR, myeloid differentiation factor 88 (MyD88) and tumor necrosis factor (TNF) receptor-associated factor 6 (TRAF6), was investigated in different developmental stages. Ontogenetic expression by real-time PCR revealed constitutive expression of these genes in all the developmental stages selected. TLR expression was found to be the highest in PL4, whereas MyD88 and TRAF6 showed the highest expression in eggs. The ubiquitous expression of TLR, MyD88 and TRAF6 in different developmental stages of P. monodon indicates the role of these genes in protecting the animals during early development. Immersion challenge of PL 18 with V. harveyi resulted in significant upregulation of TRAF6 at all time-points and significant upregulation of TLR at most of the time-points selected; however, MyD88 showed differential modulation pattern. In contrast to the bacterial challenge, WSSV infection in PL18 did not show any significant change in the expression of TRAF6, except for a downregulation observed at 12 to 48 hpi. However, TLR and MyD88 showed moderate increase in their expression, especially at late time-points. The responses of these genes to V. harveyi and WSSV immersion challenges in the juveniles (average body weight 3 g) of P. monodon were investigated in selected tissues including gill, hepatopancreas, and different parts of gastrointestinal tract such as foregut (stomach), midgut and hindgut. Temporal expression analysis revealed complete downregulation of PmMyD88 at most of the time-points in the gill following V. harveyi challenge and significant induction of PmTRAF6 at all time-points following WSSV infection. Immersion challenge with V. harveyi resulted in enhanced expression of TRAF6 in stomach and MyD88 in hepatopancreas showed similar pattern of expression post-WSSV challenge. Other tissues showed varying levels of induction of these genes at different time-points following pathogen challenge. The results of the present study suggest that both bacterial and viral challenges through immersion modulates the genes involved in the Toll pathway, and this might play an important role in the immune defense of post-larvae and juveniles of P. monodon.

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黑虎对虾(Penaeus monodon)在哈维弧菌和白斑综合征病毒(WSSV)侵染后的个体发生及Toll通路相关基因的表达
本研究研究了虎对虾(Penaeus monodon) Toll通路中TLR、髓样分化因子88 (MyD88)和肿瘤坏死因子受体相关因子6 (TRAF6)等不同先天免疫基因在不同发育阶段的个体表达情况。实时聚合酶链反应(real-time PCR)显示这些基因在所有发育阶段均有组成性表达。TLR在PL4中表达量最高,而MyD88和TRAF6在鸡蛋中表达量最高。TLR、MyD88和TRAF6在单爪虫不同发育阶段的普遍表达表明这些基因在动物早期发育过程中具有保护作用。用V. harveyi浸泡刺激pl18可导致TRAF6在所有时间点显著上调,TLR在大部分时间点显著上调;而MyD88则表现出差分调制模式。与细菌攻击相比,WSSV感染PL18没有显示TRAF6表达的任何显著变化,除了在12至48 hpi时观察到下调。然而,TLR和MyD88的表达呈中度增加,尤其是在晚些时候。这些基因的反应鳗和种浸挑战青少年(平均体重3 g)的p .他们用了在选定的组织包括鳃、肝胰腺、和胃肠道的不同部分,如前肠(胃)、中肠和后肠。时间表达分析显示,在V. harveyi攻击后,PmMyD88在鳃的大多数时间点完全下调,而在WSSV感染后的所有时间点,PmTRAF6都被显著诱导。哈维氏弧菌浸渍侵染导致胃中TRAF6的表达增强,肝胰腺中MyD88的表达在wssv侵染后表现出类似的表达模式。其他组织在病原体攻击后的不同时间点显示出不同水平的这些基因诱导。本研究结果表明,细菌和病毒通过浸泡的挑战调节Toll通路相关基因,这可能在单殖单胞单胞虫幼虫和幼虫的免疫防御中发挥重要作用。
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Agri Gene
Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)
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期刊介绍: Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.
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