Expression of lipogenic genes in the muscle of beef cattle fed oilseeds and vitamin E

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2020-03-01 DOI:10.1016/j.aggene.2019.100097

Márcio Machado Ladeira , Dalton Mendes de Oliveira , Jon Partrick Schoonmaker , Mário Luiz Chizzotti , Horllys Gomes Barreto , Luciano Vilela Paiva , Tamara Cristina Coelho , Otávio Rodrigues Machado Neto , Mateus Pies Gionbelli , Antônio Chalfun-Junior

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引用次数: 2

Abstract

Different fat ingredients and their fatty acids may influence the expression of genes responsible to encode enzymes and proteins linked to adipose tissue deposition, influencing beef quality. Therefore, the objective was to analyze the expression of PPARA, SREBF1 and genes involved in lipid metabolism in longissimus thoracis (LT) muscle of cattle fed diets containing soybean or cottonseed with or without vitamin E. Twenty-eight bulls were used in a completely randomized design using a 2 × 2 factorial arrangement. The inclusion of soybean and cottonseed in the diets provided 64.8 g kg⁻¹ and 65.6 g kg⁻¹ of ether extract, respectively, and vitamin E supplementation was 2500 IU per day. The PPARA expression was greater and SREBF1 expression was lower in the muscle of animals fed soybean (P < .01). Vitamin E reduced the expression of SREBF1 in animals fed cottonseed and increased its expression in animals fed soybean (P < .01). Expression of LPL and ACACA increased when bulls were fed with soybean (P < .01) and their expression was upregulated when animals received vitamin E (P < .03). Expression of SCD was higher when cottonseed and no vitamin E diet were used (P < .01). In conclusion, supplementation with vitamin E alters the expression of genes involved in lipid metabolism in LT muscle, indicating that α-tocopherol is a cell-signaling molecule.

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添加油籽和维生素E的肉牛肌肉中脂肪生成基因的表达

不同的脂肪成分及其脂肪酸可能影响负责编码与脂肪组织沉积相关的酶和蛋白质的基因的表达，从而影响牛肉品质。因此，目的是分析饲粮中添加或不添加维生素e的大豆或棉籽的牛胸最长肌(LT)中PPARA、SREBF1和脂质代谢相关基因的表达。28头公牛采用2 × 2因子安排，采用完全随机设计。饲粮中添加大豆和棉籽分别提供64.8 g kg−1和65.6 g kg−1的粗脂肪提取物，维生素E补充量为2500 IU / d。饲喂大豆的动物肌肉中PPARA表达量较高，SREBF1表达量较低(P < .01)。维生素E降低了棉籽饲料中SREBF1的表达，增加了大豆饲料中SREBF1的表达(P < .01)。饲喂大豆时，LPL和ACACA的表达增加(P < .01)，饲喂维生素E时，LPL和ACACA的表达上调(P < .03)。棉籽饲粮和不添加维生素E饲粮时，SCD的表达量较高(P < .01)。综上所述，补充维生素E改变了LT肌肉脂质代谢相关基因的表达，表明α-生育酚是一种细胞信号分子。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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