FABP1 and SLC2A5 expression levels affect feed efficiency-related traits

Q1 Agricultural and Biological Sciences Agri Gene Pub Date : 2020-03-01 DOI:10.1016/j.aggene.2019.100100

Wellison J.S. Diniz , Kamila O. da Rosa , Polyana C. Tizioto , Gerson B. Mourão , Priscila S.N. de Oliveira , Marcela M. de Souza , Luciana C.A. Regitano

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引用次数: 3

Abstract

Improving the efficiency of production to reduce the environmental footprints is pivotal to the sustainability of livestock systems. Despite the advances in cattle feed efficiency (FE) measurement and identification of potential mechanisms involved, much is still unclear regarding the genetic and biological basis of this trait. Nevertheless, lipid and carbohydrate metabolism have been outlined as important in determining efficient and inefficient animals. To address the role of genes partaking in these processes and previously involved with residual feed intake (RFI), we carried out a liver expression profile in Nelore steers (n = 83). Six target genes (FABP1, FADS2, PPP1R26, RGS2, SLC2A5, and UCP2) were measured by qPCR analysis. A general linear mixed model approach was applied to associate them with dry matter intake (DMI), body weight (BW), metabolic BW (MBW, kg), DMI as a percentage of BW (DMI%BW), and average daily gain (ADG, kg/d). Residual feed intake (RFI), feed conversion ratio (FCR), feed efficiency (FE), Kleiber index (KI), and relative growth rate (RGR) were also evaluated. Our results support that increased expression of FABP1 gene was associated with enhanced values for RFI and DMI. Likewise, higher expression level of SLC2A5 was related to higher KI and RGR. There was no phenotypic correlation between RFI and ADG, BW, and MBW. The positive correlations between FABP1 and SLC2A5, and between FABP1 and FADS2 gene expression suggest a putative co-regulation affecting feed efficiency phenotypes.

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FABP1和SLC2A5的表达水平影响饲料效率相关性状

提高生产效率以减少环境足迹对畜牧业系统的可持续性至关重要。尽管在牛饲料效率(FE)的测量和潜在机制的识别方面取得了进展，但关于这一性状的遗传和生物学基础仍不清楚。尽管如此，脂质和碳水化合物代谢已被概述为确定高效和低效动物的重要因素。为了研究参与这些过程并先前与剩余采食量(RFI)有关的基因的作用，我们在Nelore肉牛(n = 83)中进行了肝脏表达谱分析。采用qPCR检测6个靶基因(FABP1、FADS2、PPP1R26、RGS2、SLC2A5和UCP2)。采用一般线性混合模型方法将它们与干物质采食量(DMI)、体重(BW)、代谢体重(MBW, kg)、DMI占体重的百分比(DMI%BW)和平均日增重(ADG, kg/d)相关联。评价剩余采食量(RFI)、饲料系数(FCR)、饲料效率(FE)、Kleiber指数(KI)和相对生长率(RGR)。我们的研究结果支持FABP1基因表达的增加与RFI和DMI值的增加有关。SLC2A5表达水平越高，KI和RGR也越高。RFI与ADG、BW和MBW之间无表型相关性。FABP1与SLC2A5、FABP1与FADS2基因表达之间呈正相关，表明可能存在影响饲料效率表型的共同调控。

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Agri Gene Agricultural and Biological Sciences-Agricultural and Biological Sciences (miscellaneous)

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期刊介绍： Agri Gene publishes papers that focus on the regulation, expression, function and evolution of genes in crop plants, farm animals, and agriculturally important insects and microorganisms. Agri Gene strives to be a diverse journal and topics in multiple fields will be considered for publication so long as their main focus is on agriculturally important organisms (plants, animals, insects, or microorganisms). Although not limited to the following, some examples of potential topics include: Gene discovery and characterization. Genetic markers to guide traditional breeding. Genetic effects of transposable elements. Evolutionary genetics, molecular evolution, population genetics, and phylogenetics. Profiling of gene expression and genetic variation. Biotechnology and crop or livestock improvement. Genetic improvement of biological control microorganisms. Genetic control of secondary metabolic pathways and metabolic enzymes of crop pathogens. Transcription analysis of beneficial or pest insect developmental stages Agri Gene encourages submission of novel manuscripts that present a reasonable level of analysis, functional relevance and/or mechanistic insight. Agri Gene also welcomes papers that have predominantly a descriptive component but improve the essential basis of knowledge for subsequent functional studies, or which provide important confirmation of recently published discoveries provided that the information is new.

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