Investigating the Effect of Fennel and Cinnamon Combined Extract on Spermatogenesis and Testis Tissues in Busulfan Induced Infertile Rats

Q3 Biochemistry, Genetics and Molecular Biology Journal of Applied Biotechnology Reports Pub Date : 2019-09-11 DOI:10.29252/JABR.06.03.03

R. Chegini, P. Soleimani, M. Sadeghi, Rana Mohammad Yosef, F. Zafari

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引用次数: 1

Abstract

Introduction: Busulfan is one of the common cancer treatment drugs with infertility side effects. Fennel and cinnamon are two medicinal plants with fertility enhancement properties. The aim of this study was to investigate the effects of fennel and cinnamon on busulfan induced infertile rats. Materials and Methods: Forty male Wistar rats were divided into four groups including: sham group: healthy rats without intervention, control group: Busulfan treated rats, fennel group: busulfan and fennel extract treated, fennel and cinnamon group: busulfan, fennel and cinnamon extract treatment. Testicular tissues were sampled and the testicular physical parameters and spermatogenesis level were evaluated by H & E staining and optical microscopy imaging. Results: The biggest and the smallest testis lengths were observed in cinnamon + fennel and fennel groups respectively (P < 0.05). The highest and lowest sperm levels were observed in the cinnamon + fennel group and fennel group respectively (P < 0.001). The total average of reproductive cells was the most in the cinnamon + fennel group (104.17) and had the least level in the control group (26.29). Conclusions: The combined extract of fennel and cinnamon significantly protect the testicular tissues against infertility effect of busulfan. However, the fennel extract alone increased the effect of busulfan in rats.

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茴香肉桂联合提取物对布苏凡诱导不育大鼠精子发生及睾丸组织影响的研究

简介:布磺胺是一种常见的有不孕副作用的癌症治疗药物。茴香和肉桂是两种具有增强生育力的药用植物。本研究旨在探讨茴香和肉桂对布苏凡诱导的不育大鼠的影响。材料与方法:将40只雄性Wistar大鼠分为4组:假手术组:未干预的健康大鼠，对照组:丁磺凡治疗大鼠，茴香组:丁磺凡加茴香提取物治疗大鼠，茴香加肉桂组:丁磺凡加茴香提取物治疗大鼠，茴香加肉桂提取物治疗组。取睾丸组织标本，通过h&e染色和光学显微镜成像观察睾丸物理参数和精子发生水平。结果:肉桂+茴香组和茴香组睾丸长度最大、最小(P < 0.05);肉桂+茴香组和茴香组小鼠精子水平最高、最低(P < 0.001)。生殖细胞总数以桂皮+茴香组最多(104.17)，对照组最少(26.29)。结论:茴香肉桂联合提取物对大鼠睾丸组织有明显的保护作用。而茴香提取物在大鼠体内的作用增强。

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来源期刊

Journal of Applied Biotechnology Reports Biochemistry, Genetics and Molecular Biology-Biotechnology

CiteScore

1.90

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0.00%

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期刊介绍： The Journal of Applied Biotechnology Reports (JABR) publishes papers describing experimental work relating to all fundamental issues of biotechnology including: Cell Biology, Genetics, Microbiology, Immunology, Molecular Biology, Biochemistry, Embryology, Immunogenetics, Cell and Tissue Culture, Molecular Ecology, Genetic Engineering and Biological Engineering, Bioremediation and Biodegradation, Bioinformatics, Biotechnology Regulations, Pharmacogenomics, Gene Therapy, Plant, Animal, Microbial and Environmental Biotechnology, Nanobiotechnology, Medical Biotechnology, Biosafety, Biosecurity, Bioenergy, Biomass, Biomaterials and Biobased Chemicals and Enzymes. Journal of Applied Biotechnology Reports promotes a special emphasis on: -Improvement methods in biotechnology -Optimization process for high production in fermentor systems -Protein and enzyme engineering -Antibody engineering and monoclonal antibody -Molecular farming -Bioremediation -Immobilizing methods -biocatalysis